WGU C785 BIOCHEMISTRY FINAL OA EXAM 2023-2024
REAL EXAM QUESTIONS AND CORRECT
ANSWERS|AGRADE(COMPLETE EXAM)
- What is the complementary sequence to 3’ ATG CGAATA 5’? *
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3’ TAT TCG CAT 5’
3’ TAC GCT TAT 5’
3’ GAT AGC ATA 5’
5’ATAAGC GTA 3’ - Which amino acid sequence would result from this Coding DNA
strand? 3’ ATG CAG ATA 5’? *
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Feedback
The correct answer is 3′ TAT TCG CAT 5′. Remember complementary means “Matching or Pairing”
You have to remember to pay attention to your numbers as well as your letters (A-T, G-C, 5′-3′). The
correct answer is
3’ ATG CGA ATA 5’ (original sequence)
5’ TAC GCT TAT 3’ (complementary sequence)
But we asked for it in the 3’ direction, so mirror answer to give correct answer:
3’ TAT TCG CAT 5’
Tyr Val Tyr
Ile Gln Ile
Ile Asp Val
Leu His Lys
- Which amino acid sequence would be made from this template
DNA strand? 5’ TAT TAC CGA 3’? *
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Feedback
The correct answer is Ile Asp Val. We are starting at the coding strand, and have to remember the
relationship between coding DNA and mRNA. These two strands are non-complementary and
parallel. So we copy the coding strand , change T —> U, and then write the mRNA sequence:
3’ ATG CAG ATA 5’ coding
3’ AUG CAG AUA 5’ mRNA
Mirror by changing orientation: 5’ AUA GAC GUA 3’
Read chart Ile Asp Val (chart is in direction of 5′ —> 3′)
Tyr Tyr Arg
Ile Met Ala
Ser Val Ile
Ser His Gln
- Which of the following are the correct components for a PCR
reaction? *
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dNTPs, Primer, RNA Polymerase, template RNA
dNTPs, Primer, DNA Polymerase, template DNA
ATP, Primer, mRNA polymerase; template mRNA
Correct answer
Ser Val Ile
Feedback
The correct answer is C because
5’ TAT TAC CGA 3’ template is complementary and antiparallel so
3’ AUA AUG GCU 5’ but it is in the wrong orientation, so mirror
5’ UCG GUA AUA 3’ and read the chart
Ser Val Ile
Acetyl CoA, RNA primer, DNA Ligase, Template phosphate
- If you had a mother who is homozygous for a recessive disease,
and a father who is a heterozygous carrier, what percentage of
children will have the disease? *
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0%
25%
50%
75%
100% - Which of the following would represent a silent mutation if this is the
original sequence: 5’ AUC GUA ACA 3’? *
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Feedback
The correct answer is c 50%
Homozygous recessive: aa
Heterozygous: Aa
Feedback
The correct answer is b