MLT(ASCP) Exam ASCP-MLT Questions

  1. The red portion of the NFPA diamond represents
    fire hazards. The fire classification system for NFPA is
    as follows:
    0 – Will not burn
    1 – Must be preheated for ignition; flashpoint above 200°F (93°C)
    2 – Must be moderately heated for ignition, flashpoint above 100°F (38°C)
    3 – Ignition may occur under most ambient conditions, flashpoint below
    100°F (38°C) 4 – Extremely flammable and will readily disperse through
    air under standard conditions, flashpoint below 73°F (23°C)
    A laboratory labels its secondary containers of hazardous chemicals using
    a color- coded system, with a different color representing each different
    type of hazard. The coding system follows the color-coding used by the
    National Fire Protection Agency’s “fire diamond.” What hazard would be
    represented by the red section on the hazard label?
    A. Health hazard
    B. Fire hazard
    C. Reactivity hazard
    D. Special
    hazards Answer:
    B
  2. Acute hemolytic transfusion reactions are most commonly due to ABOincompatible blood being transfused to a recipient with naturally occurring
    ABO alloantibodies (anti- A, anti-B, anti A,B).
    Acute intravascular hemolysis as the result of a blood transfusion is
    most often associated with which of the following causes?
    A. Transfusion of ABO incompatible red cells
    B. Allergies
    C. Passively transfused antibodies to HLA antigens
    D. Transfusion-associated graft-versus-host
    disease Answer: A
    3.
    4.B
  3. D
    6.A
    7.C
    Red to Brown Urine: porphobilinogen, hematuria,
    myoglobinuria, etc. Green: Food colorings; Increased
    carotene in the diet;
    Pseudomonas aeruginosa
    infection Yellow: bilirubin, bile
    pigments White: phosphates,
    other crytals
    Match urine color with substance that might have been responsible:
  4. Phosphates
    9.Bilirubin
    10.Pseudomonas
    11.Porphobilinogen
    A. Blue to green
    B. White
    C. Red to brown
    D. Yellow
    Answer:
    A,B,C,D
  5. Hybridization is a technique used to determine presence of target
    DNA or RNA by adding a synthetic strand that binds by complementary
    base pairing to the target.
    Microbiology
    In molecular diagnostic testing, hybridization employs
    complementary base pair binding of a synthetic strand to DNA or
    RNA.
    A. true
    B. false
    Answer:
    A
  6. Caffeine benzoate solution is used to split the unconjugated bilirubin
    protein complex releasing the bilirubin so that it can react with diazotised
    sulphanilic acid. The tartrate buffer creates an alkaline solution and
    converts the red acid bilirubin to a green coloured compound which can
    be measured spectrophotometrically.
    Which substance is used in the Jendrassik-Grof method to accelerate the
    reaction of unconjugated bilirubin with the diazo reagent?
    A. NADH
    B. N-butanol
    C. caffeine
    D. acetic

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