BACE Exam Review Questions With
Complete Solutions
bioethics – Answer applying moral values to decision-making
on topics brought up by advances in biology, medicine, and
technology
biotechnology – Answer the study and manipulation of living
things or their components
data – Answer information gathered during an experiment
genome – Answer an entire set of an organism’s genetic
information
hypothesis – Answer a testable prediction to a scientific
question
journal – Answer a scientific magazine or periodical where
scientists publish their experiments
control – Answer an experimental trial with a factor that will
yield a predictable result
morals – Answer values that help one decide between right and
wrong
recombinant DNA – Answer DNA from 2 or more different
source organisms in one DNA molecule
variable – Answer a factor that gets manipulated during an
experiment
amino acid – Answer the subunits of proteins, each of which
contains an amino group, carboxyl group, and a functional “R”
group all attached to a central carbon atom
carbohydrate – Answer molecules containing carbon, hydrogen,
and oxygen in approximately a 1:2:1 ratio
chromosomes – Answer a long DNA molecule wrapped around
histone proteins
glucose – Answer a six-carbon sugar molecule used by cells for
making energy
lipids – Answer hydrophobic macromolecules including fats,
oils, and steroids
macromolecules – Answer large molecules composed of
repeating units of smaller molecules
nucleic acids – Answer information-carrying molecules
composed of nucleotides
organelles – Answer specialized membrane-bound structures
that have specialized functions within cells
photosynthesis – Answer a process where plants and algae use
light energy to make chemical energy
BACE EXAM STUDY GUIDE QUESTIONS
WITH COMPLETE SOLUTIONS
What are the Common Career Technical Core – Career Ready
Practices? – Answer 1. Act as a responsible and contributing
citizen and employee.
- Apply appropriate academic and technical skills.
- Attend to personal health and financial well-being.
- Communicate clearly, effectively and with reason.
- Consider the environmental, social and economic impacts of
decisions. - Demonstrate creativity and innovation.
- Employ valid and reliable research strategies.
- Utilize critical thinking to make sense of problems and
persevere in solving them. - Model integrity, ethical leadership and effective management.
- Plan education and career path aligned to personal goals.
- Use technology to enhance productivity.
- Work productively in teams while using cultural/global
competence.
What is the scientific method and what are its steps? – Answer
The scientific method is a process for experimentation that is
used to explore observations and answer questions. - identify the problem
- ask questions
- research the questions
- create a hypothesis
- conduct experiments
- collect and analyze data
- draw conclusions
- share the results
What is PCR (polymerase chain reaction)? – Answer
Polymerase chain reaction, or PCR, is a technique to make many
copies of a specific DNA region in vitro (in a test tube rather
than an organism).
Amplifies a target region quickly & cost effectively
What is used in PCR? – Answer PCR relies on a thermostable
DNA polymerase, Taq polymerase, and requires DNA primers
designed specifically for the DNA region of interest.
How is PCR done? – Answer
What are the applications of PCR? – Answer
- Answer
What is the role of the mitochondria? – Answer
Where is DNA stored in a eukaryotic cell? – Answer
What is the difference between a eukaryotic and a prokaryotic
cell? – Answer
What is the role of the Golgi apparatus? – Answer Consists of a
stack of curved saccules that receives protein and also lipidfilled vesicles from the ER, packages, processes, and distributes
them within the cell
BACE Exam-Knowledge Questions With
Complete Solutions
Clinical Trial- Phase 1 – Answer – Tests drug in healthy
individuals
- Tests for safety, dosage, and side effects
Clinical Trial- Phase 2 – Answer – Tests on larger group of
effected individuals - Tests for efficacy and side effects
Clinical Trial-Phase 3 – Answer – Tests on new and wider
demographic - Tests for long term effectiveness and comparisons with other
medications
Clinical Trial- FDA Approval – Answer Treatment determined
effective and safe for public use
Clinical Trial- Phase 4 – Answer – Continues to test for
effectiveness and safety - Can be taken off the market if necessary
Cell Harvesting- Indirect Cell Count – Answer Determine
concentration of cells is a liquid culture
DOES NOT tell me if the cells are viable vs nonviable- some
cells may be dead
Cell Harvesting- Indirect Cell Count Reagents – Answer – Luria
Broth(Food Source) - Shaking Incubator or shaking water bath (Incubator or Water is
for 37 C; Shaking-Oxygen) - Spectrophotometer (measures absorbance values to give
concentration)
Cell Harvesting- Direct Cell Count – Answer Determine
concentration of cells in agar place
Cell Harvesting- Direct Cell Count Reagents – Answer – Agar
Plate - Luria Broth(food source)
- Incubator (37 C)
- Physically Count- the amount the colonies and calculate the
concentration
Gram Staining – Answer Determine if peptidoglycan is present
in cell wall
Gram Staining Reagents – Answer – Crystal Violet=Gram
Positive - Iodine= fixation
- Alcohol= decolorizer
- Safranin= Gram negative
Inserting into the Cell – Answer Neutralize cell membrane and
substance being inserted now passive disuse can occur
Inserting into the Cell- Reagents – Answer – Cofactors: CaCl2
or MgCl2 - Heat-shock (chemical transformation= prokaryotic) or
Electricity/Electroporation (Transfection= Eukaryotic Cells)
BACE Practice Exam Questions Version A
Questions With Complete Solutions
NaCl is a:
-nonpolar covalent
-ionic
-polar covalent – Answer ionic
H2O is a:
-nonpolar covalent
-ionic
-polar covalent – Answer polar covalent
CH4 is a:
-nonpolar covalent
-ionic
-polar covalent – Answer nonpolar covalent
What should be used to calculate the amount of compound
required to make a solution? – Answer molecular weight
What is an example of a carbohydrate and its associated
functions? – Answer Example is cellulose. Functions are
energy storage, receptors, structure of plant cell wall
What is an example of a lipids and its associated functions? –
Answer Example: cholesterol; Functions: membrane, structure,
energy storage
What is an example of a nucleic acids and its associated
functions? – Answer Example is RNA, it functions: information
storage and transfer
What is an example of a proteins and its associated functions? –
Answer Example is enzymes, its functions: structure, receptors,
transport
True or False
A solvent is the dissolved matter in a solution – Answer False
When microorganisms (usually yeast) undergo fermentation,
they obtain their energy by: – Answer Anaerobic respiration
The enzyme maltase catalyzes the break down of maltose into
glucose. A technician added maltase to a solution of maltose to
be incubated at 37°C. Samples were taken from the reaction tube
every five minutes over the course of one hour, to measure the
glucose concentration. After an hour, the technician noticed the
water bath failed to reach 37°C (it only warmed to 25°C).
Which of the following is the most likely outcome of the
reaction? – Answer The enzyme activity would decrease,
yielding the same level of glucose over a longer time period.
How does DNA differ from one species to another? – Answer
The number of genes and non-coding regions
What type of DNA (and description) is circular, long DNA
containing several thousand genes – Answer Prokaryotic DNA
BACE Practice Exam Version A 3.0 General
Topics in Biotechnology Questions With
Complete Solutions
Which of the following is NOT considered a significant advance
in PCR methodology? – Answer the use of agarose gel
electrophoresis to visualize amplified products
Match the enzyme to its potential use in the laboratory
DNA ligase – Answer joining pieces of DNA together
Match the enzyme to its potential use in the laboratory
Heat-stable DNA polymerase – Answer preforming PCR
reactions
Match the enzyme to its potential use in the laboratory
Protease – Answer degrading protein
Match the enzyme to its potential use in the laboratory
Restriction enzymes – Answer digesting DNA
It is debated that genetically modifies food crops have both
beneficial and negative effects on humans and on the
environment. Which of the following is a potential negative
outcome of growing or consuming genetically modified food
crops? – Answer -Genetically modified crops may crosspollinate with non-genetically modified crops
-Growing only genetically modified crops my decrease genetic
diversity
Which of the following is an advantage of treating diabetic
patients with genetically engineered human insulin, rather than
pig or cow insulin? – Answer -There is less chance of an
immune response to human insulin
-Ethical issues concerning the use of animals are avoided
-It is more efficient process to purify insulin from yeast or
bacterial cultures than it is from and animal organs
“Bioethics” is best described as: – Answer the study of decision
making as it applies to moral decisions that must be made sue ti
advances in biology and technology
Why might some people be ethically opposed to using
CRISPR/Cas9 to create mutations in animal embryos? – Answer
Alterations in germline cells will be passed on to future
generations
Match the person or group with the best example of their
responsibility in a biotechnology company.
Ensure that all government regulations are followed, working
closely with all departments – Answer Quality Assurance (QA)
Match the person or group with the best example of their
responsibility in a biotechnology company.
BACE Practice Review Questions With
Complete Solutions
A solvent is the dissolved matter in a solution False
The enzyme maltase catalyzes the break down of maltose into
glucose. A technician added maltase to a solution of maltose to
be incubated at 37°C. Samples were taken from the reaction tube
every five minutes over the course of one hour, to measure the
glucose concentration. After an hour, the technician noticed the
water bath failed to reach 37°C (it only warmed to 25°C).
Which of the following is the most likely outcome of the
reaction? The enzyme would become inactive, yielding no
glucose
Circular, long DNA containing several thousand genes
Prokaryotic DNA
Linear, long pieces of DNA with many thousands of genes,
contained within a nucleus Eukaryotic DNA
Which of the following serves as the decolorizer in a Gram
stain? Alcohol
Which of the following is an accurate description of Grampositive bacteria after Gram staining? Purple, because Grampositive bacteria have a thick layer of peptidoglycan in the cell
membrane.
Which of the following is NOT considered a significant advance
in PCR methodology? The use of agarose gel electrophoresis
to visualize amplified products
Heat-stable DNA polymerase Performing PCR reactions
Protease Degrading protein
Restriction Enzymes Digesting DNA
Why might some people be ethically opposed to using
CRISPR/Cas9 to create mutations in animal embryos?
Alterations in germline cells will be passed on to future
generations.
Which of the following is the study of the entire set of proteins
of a cell or organism? Proteomics
Steps of the production of a product Quality Control test and
approves
Manufacturing produces the product following a batch record
Quality Control tests the final product following test records
Quality Assurance approves or rejects the final product based on
final product specifications
Maintaining a safe work environment is critical for the wellbeing of all employees. Which of the following agencies i
BACE Practice Test 2 Questions with
Complete Solutions
Complimentary base pairing between two DNA strands is due to
the formation of – Answer hydrogen bonds
Cellulose is an important raw material for textiles and a variety
of other industries. Which of the following is a function of
polysaccharides in the cell? – Answer Providing structure and
energy storage within cells
Lipids are composed mainly of which of the following atoms? –
Answer carbon and hydrogen
T/F: A solute is the dissolved matter in a solution. – Answer
true
Process which uses oxygen as a raw material – Answer aerobic
respiration
Process which releases carbon dioxide and ethyl alcohol –
Answer anaerobic respiration
Process by which glucose is utilized by a cell – Answer Both
Aerobic and Anaerobic Respiration
Process which uses carbon dioxide as a raw material – Answer
Neither Aerobic or Anaerobic Respiration
Why are enzymes necessary in living organisms? – Answer
Enzymes increase the rate of chemical reactions which would
otherwise occur too slowly to sustain life.
Which of the following affects the rate of a chemical reaction? –
Answer Concentration, Heat, Presence of a Catalyst
Made of four nitrogenous bases – Answer all DNA
Contained in a nucleus – Answer Eukaryotic DNA
Long, circular DNA with several thousand genes – Answer
Prokaryotic DNA
Small, circular DNA floating in the cytoplasm – Answer
Plasmid
Double helix structure of repeating nucleotides – Answer All
DNA
Linear, very long pieces of DNA with many thousands of genes
- Answer Eukaryotic DNA
When DNA replicates, each original strand of DNA serves as a
template for a new strand. What type of replication is this? –
Answer Semi-conservative replication
Transcription begins when RNA polymerase binds to which area
on the DNA strand? – Answer Promoter
Many enzymes used in biotechnology applications occur
naturally within the cell. Which enzyme is involved in DNA
replication? – Answer DNA Polymerase
Where does translation take place in prokaryotes? – Answer
ribosomes
If a bacterial cell did not contain restriction enzymes, which of
the following would be likely to occur? – Answer The cell
would be easily infected and lysed by bacteriophages.
Cell theory states: – Answer All life forms are made from one
or more cells.
Cells only arise from pre-existing cells.
The cell is the smallest form of life.
What happens at the ribosome? – Answer protein synthesis
Which type of cell could use sugar to produce energy in an
oxygen-deprived environment? – Answer bacterial, animal
(muscle), yeast
After mitotic division, how many chromosomes do daughter
cells have compared with the parent cell? – Answer the same
number
Which of the following is an accurate description of Gram
positive bacteria after Gram staining? – Answer Purple,
because gram positive bacteria have a thick layer of
peptidoglycan in the cell wall.
BACE Tests Questions With
Complete Solutions
what represents the correct order of assembly in the cell –
Answer Amino acid, polypeptide, protein
t/f: A single base pair change in a gene’s DNA sequence can
affect the shape and function of the protein it encodes. – Answer
true
t/f:The three-dimensional structure of a protein is largely
determined by the amino acid sequence. – Answer true
A structural gene’s code is 5′ TAC ATG CCC TTA ATC 3′,
what will the mRNA transcript be? – Answer 3′ AUG UAC
GGG AAU UAG 5′
Circular, long DNA containing several thousand genes – Answer
Prokaryotic DNA
Cytoplasmic, circular DNA containing only a few genes –
Answer plasmid DNA
Linear, long pieces of DNA with many thousands of genes,
contained within a nucleus – Answer Eukaryotic DNA
How does DNA differ from one species to another? – Answer
The number of genes and non-coding regions
The enzyme maltase catalyzes the break down of maltose into
glucose. A technician added maltase to a solution of maltose to
be incubated at 37°C. Samples were taken from the reaction tube
every five minutes over the course of one hour, to measure the
glucose concentration. After an hour, the technician noticed the
water bath failed to reach 37°C (it only warmed to 25°C).
Which of the following is the most likely outcome of the
reaction? – Answer The enzyme activity would decrease,
yielding the same level of glucose over a longer time period.
Carbon dioxide as a by-product benefits the brewing and baking
industry in a process called fermentation. When microorganisms
(usually yeast) undergo fermentation, they obtain their energy
by: – Answer Anaerobic respiration
t/f:A solvent is the dissolved matter in a solution. – Answer
false
Carbohydrates – Answer Example: cellulose. Functions: energy
storage, receptors, structure of plant cell wall.
Lipids – Answer Example: cholesterol. Functions: membrane
structure, energy storage.
Nucleic acids – Answer example: RNA. Functions: information
storage and transfers
Proteins – Answer example: enzyme. Function: structure,
receptors and transport
Biotech BACE Exam Questions With
Complete Solutions
The process of making RNA from DNA – Answer
Transcription
The process by which RNA is decoded and polypeptides are
formed – Answer Translation
Autonomously replicating small circles of DNA present in some
bacteria – Answer Plasmids
Modifications of DNA in an organism – Answer Genetic
engineering
Parkinson’s, diabetes, and cystic fibrosis (CF) are examples of –
Answer Genetic disorders
Organism that does not have nucleus or membrane bound
organelle, so DNA in contained in cytoplasm – Answer
Prokaryotes
Help researchers measure the amount of protein present –
Answer Concentration assays
Determines the amount and length of activity for a given
enzyme or drug in a test organism – Answer Potency assays
Tests that show if a specific enzyme or drug is present and
conducting the expected reaction – Answer Activity assays
A highly specific test to determine protein concentration based
in a particular antigen-antibody interaction – Answer ELISA
Test used for understanding the relationship between dosage and
activity of a given pharmaceutical drug – Answer PK/PD
assays
Tests for determining the presence or a particular characteristic
of a protein of interest – Answer Assays
An aqueous solution and yields hydrogen ion when ionized –
Answer Acid
A solution that resists changes in pH – Answer Buffer
The measurement of the concentration of hydrogen ion in a
sample – Answer pH
The separation of molecules on or through a stationary phase –
Answer Chromatography
Quantitative study of electromagnetic spectra deals with visible
light, near-ultraviolet, and near-infrared light source – Answer
Spectrophotometry
Form of liquid chromatography used to separate compounds that
are dissolved in solution. Instruments consist of a reservoir of
mobile phase, a pump, an injector, a separation column, and a
detector. – Answer HPLC
Biotility Bace practice knowledge Exam
Questions With Complete Solutions
cell theory states – Answer All life forms are made from one or
more cells. Cells only arise from pre-existing cells. The cell is
the smallest form of life.
E.coli is an example of a eukaryote – Answer false
Quality Assurance – Answer ensure that all products meet
standards of quality in manufacturing process
reasearch and development (R AND D) – Answer identify
candidate biopharmaceutical drugs
Manufacturing – Answer make commercial quantities of
biopharmaceutical drug
Which structure regulates the flow of molecules into and out of
the cell? – Answer Plasma Membrane
Which bacterial cell component is commonly isolated and used
to digest DNA? – Answer Restriction Enzyme
polymerase chain reaction ( PCR) – Answer Heat-stable DNA
polymerase
Digesting DNA – Answer Restriction enzymes ( EcoRI)
Running DNA gels – Answer Horizontal gel electrophoresis
box
Running protein gels ( PAGE) – Answer Vertical gel
electrophoresis box
Joining pieces of DNA – Answer DNA ligase
Spectrophotometer – Answer Measuring protein or DNA
concentration
A technician reapeated the maltase reaction described above, but
the water bath failed to reach 37°C ( it is warmed to 30°C).
What is the most likely out come? – Answer The enzyme
activity would decrease , yielding the same level of glucose over
a longer time period.
Reaguarding the out come in question 8 , select the most
appropriate action for the technician. – Answer Report the
problem to QA or individual in charge of repairs and place an
out of service lable on the water bath
The byproduct of carbon dioxide benefits the brewing and
baking industries. carbon dioxide is produced by
microorganisms ,usually yeast, that obtain their energy by: –
Answer Anaerobic Respiration
Where is the energy in the triphosphate (ATP) ? – Answer
Phosphate bonds
In a DNA double helix, the two DNA chains are held together
by: – Answer Hydrogen bonds between the pair of bases
3.0 BACE Exam Questions With
Complete Solutions
The invention of the thermal cycler
The use of reverse transcriptase from single-stranded RNA
The use of fluorescently-tagged probes for real-time quantitation
of PCR products – Answer SIgnificant Advances in PCR
include
the structure of DNA was described, as well as its method of
replication – Answer What happened in the 1950s?
the isolation of restriction enzymes – Answer The advent of
recombinant DNA technology began with what?
it allowed us to manipulate DNA – Answer What did the
advent of recombinant DNA technology do ?
Vaccines – Answer They are typically made of a weakened or a
killed form of a disease-causing microorganism.
Vaccination function – Answer They signal the immune system
to make antibodies against the microorganism for which the
vaccine was developed.
Vaccine protection – Answer They can provide protection
against future infection by the pathogen for which they were
developed.
Restriction Enzymes – Answer digest DNA
DNA Ligase – Answer join pieces of DNA together
Heat-stabled DNA polymerase – Answer performs reactions in
PCR
Protease – Answer degrades proteins
developing medications – Answer Genetic engineering can help
in what?
Proteomics: – Answer the study of the entire set of proteins of a
cell or organism
The nutritional value of a genetically modified food crop may
increase. Genetically modified crops may be more hardy. –
Answer Pros to Genetically modified crops (transgenic crops)
Genetically modified crops may cross-pollinate with nongenetically modified crops.
Growing only genetically modified crops may decrease genetic
diversity. – Answer Cons to Genetically modified crops
(transgenic crops)
Genetically engineered animals benefits – Answer Enhanced
animal welfare and health to increase resistance to disease,
minimizing the need for animal care interventions
Production of valuable industrial products, such as spider silk
used for medical and defense purposes
Efficient production of replacement proteins, drugs, vaccines or
tissues for treating and preventing human diseases
BACE EXAM 4.0 LABORATORY SKILLS
QUESTIONS WITH COMPLETE
SOLUTION
Disinfection – Answer the process of cleaning something,
especially with a
chemical, in order to destroy bacteria.
an area
object
or person – Answer Decontamination is the removal of
dangerous substances, radioactivity or germs from…?
dangerous substances
radioactivity
and germs – Answer Decontamination is the removal of what?
Aseptic technique assures that you don’t introduce contaminants
into
a specimen and that infectious agents are not spread to you or
laboratory surfaces – Answer What is the purpose of aseptic
technique?
Technicians practice aseptic technique when culturing cells –
Answer When do technicians practice aseptic technique?
to ensure
they are growing pure cultures of their desired cells. – Answer
Why do technicians practice aseptic technique?
to place the sterile loop into the culture tube by moving the
tube over the loop – Answer When transferring bacteria using
aseptic technique, it is common practice to do what?
rather than moving the loop into the tube. – Answer When
transferring bacteria using aseptic technique, it is common
practice to place the sterile loop into the culture tube by moving
the tube over the loop, rather than what?
Sterilization – Answer destroys all microorganisms
Decontamination – Answer the neutralization or removal of
dangerous
substances, radioactivity, or germs from an area, object, or
person.
Alcohol – Answer What should not be used to disinfect an area
that is potentially contaminated by DNA?
the fine focus knob – Answer When using a microscope on the
highest power
objective, you should use….?
bright field microscope – Answer Objectives are located
beneath the stage
inverted microscope – Answer Light source is located above
the specimen
Confocal microscope – Answer Uses a laser light to scan
samples that have been dyed
Electron Microscope – Answer Uses electrons to scan
samples in near-vacuum conditions
1,000 – Answer A solution with a pH of 3.0 is how
many times more acidic than a
solution with a pH of 6.0?
logarithmic – Answer The pH scale is _ using a buffered solvent at a specific pH – Answer How are enzyme solutions always prepared? because a buffered solvent is resistant to fluctuations in pH – Answer Why are enzyme solutions always prepared using a buffered solvent at a specific pH? because it is made with a combination of a weak acid and its conjugate base – Answer Why are buffered solvents resistant to fluctuations in pH? narrow pH range – Answer Enzymes best retain their function within a _ range?
anion exchange column chromatography – Answer DNA is
purified using what?
because DNA is negatively charged, binds to the positively
charged
BACE Exam Practice Test Questions with
Complete Solutions
What is an example of a product easily found in nature? –
Answer Antibiotic
In a biotechnology lab, what is tap water is best used for? –
Answer Washing glassware.
What is the order of amino acids on a polypeptide chain encoded
by? – Answer mRNA
How do DNA molecules vary from one species to another? –
Answer Number of genes and non-coding regions.
What is agar? – Answer Solid media on which bacteria grow.
What part of an amino acid differs from one to the other? –
Answer R group
How can colorless proteins be monitored during
electrophoresis? – Answer PAGE loading dye.
Which of the following is an mRNA codon?
a. ATA
b. UAU
c. Tyrosine
d. All of the above – Answer UAU
What is the purpose for SDS during electrophoresis? – Answer
Denature the proteins and give them a negative charge.
Which governmental agency regulates biopharmaceutical
products for human use? – Answer Food & Drug
Administration (FDA)
What are the most common molecules in cells? – Answer
Proteins.
Glucose is a . – Answer Monosaccharide. Where does translation occur in prokaryotes? – Answer At the ribosomes. What is the process of making RNA from DNA? – Answer Transcription. What is the process by which RNA is decoded and polypeptides are formed? – Answer Translation. What is autonomously replicating small circles of DNA present in some bacteria called? – Answer Plasmids What are modifications of DNA in an organism called? – Answer Genetic engineering. Parkinson’s, diabetes, and cystic fibrosis (CF) are examples of _. – Answer Genetic disorders.
BACE Exam practice: Multiple Choice
Questions With Complete Solutions
how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA
(w/v)? – Answer (50 mL)(5 mg,mL) = 250 mg
a technician is assigned the task of inserting a gene, LanA, into a
plasmid to ultimately be expressed as a therapeutic protein in E
coli. label the following steps (1-4) in the correct order the
technician must perform them to accomplish her task. – Answer
1) design primers to amplify the lanA sequence using
polymerase chain reaction (PCR) and perform the PCR reaction
2) digest and provide lanA PCR product and the plasmid with
appropriate restriction enzymes
3) use ligase to “paste” LanA into a plasmid
4) transform E. coli with plasmid containing LanA
how much tetracycline is needed to prepare a 100 mL of 100 mg
/ml tetracycline (w/v)? – Answer (100mL)(100mg/ML) =
10000mg (10g)
after performing the PCR reaction with the primers she designed
to amplify lanA, the same technician found her PCR reaction
contained two distinct bands, one at the expected size of 1200
kilobase pairs, and another unexpected band at 2000 Kilobase
pairs. what is the most likely reason there’s an unexpected bands
and her PCR reaction? – Answer she used an annealing
temperature that was too low
in a biotechnology lab, tap water is best to use for: – Answer
first rinse when washing glassware
the order of amino acids on a polypeptide chain is encoded by: –
Answer mRNA
how do DNA molecules vary from one species to another? –
Answer the number of genes and non-coding regions
what is agar? – Answer solid media on which bacteria grows
what part of an amino acid differs from one to the other? –
Answer R group
how are the proteins in fractions collected during column
chromatography visualized? – Answer using polyacrylamide
gel electrophoresis (SDS PAGE)
which of the following is an mRNA codon? – Answer UAU
what is the purpose of SDS when used in SDS page
electrophoresis? – Answer denature the proteins and give them
a negative charge
which department is in charge of testing biopharmaceutical
products to ensure its safety and potency? – Answer quality
control (QC)
BACE EXAM QUESTIONS WITH
COMPLETE SOLUTIONS
4.651 x 10^4 written in expanded form is – Answer 46,510
What is 5.78 x 3.13, rounded to the correct number of significant
digits? – Answer 18.1
What is 4.202 x 0.806, rounded to the correct number of
significant digits? – Answer 3.39
How many grams is 2.6 kg? – Answer 2600
How many milliliters is 0.725L?
Group of answer choices – Answer 725
Your supervisor provides you with a 1.0 mg/mL solution of
Bovine Serum Albumin (BSA), and asks you to make a 5-step 2-
fold serial dilution. What would be the dilution factor at step 3?
- Answer 1:8
You are given a 5.0 mg/mL protein standard solution, and asked
to prepare a 5-step, 2-fold serial dilution to create a standard
curve. What is the final concentration of protein standard in
mg/mL? Record the correct amount to three decimal places. –
Answer 0.156
You are given a 5.0 mg/mL protein standard solution, and asked
to prepare a 5-step, 2-fold serial dilution to create a standard
curve. After the third step of the dilution, what is the
concentration of the protein standard, in mg/mL? Record the
correct amount to two decimal places. – Answer 0.63
Your supervisor provides you with a 1.0 mg/mL solution of
Bovine Gamma Globulin (BGG). What is the dilution factor if
you add 150 µL of 1.0 mg/mL BGG to 150 µL of diluent? –
Answer 1:2
A technician is asked to prepare a dilution of a common
laboratory disinfectant. The label indicates that a 1:29 dilution in
water is required prior to use. Using the simple dilution method,
calculate the volume in mL of disinfectant required to make 1.0
L. Record the answer to one decimal place below – Answer
34.5
An SOP for preparing a bleach solution instructs to dilute the
bleach 1:15 in water. Using the simple dilution method,
calculate the volume in mL of bleach required to make 3.5 L.
Record the correct amount to one decimal place. – Answer
233.3 mL
You are given a stock protein solution with a concentration of
24 mg/mL. Determine the protein concentration of a solution
made by mixing 5 μl of the stock with 45 μl of a buffer. Record
the amount to one decimal place. – Answer 2.4
Calculate the volume in mL of 50X TE Buffer required to make
1.5 L of 1X TE Buffer. – Answer 30 mL
Calculate the volume in mL of 25X TAE Buffer required to
make 5.0 L of 1X TAE Buffer. Record the correct amount. –
Answer 200 mL
Calculate the volume in mL of a 10.5 mg/mL tetracycline stock
solution required to make 525 mL LB with a final concentration
of tetracycline at 2.75 mg/mL. Record the answer to one decimal
place. – Answer 137.5
Which of the following is the correct method to make 1 L of
10% sodium chloride? – Answer Dissolve the appropriate
amount of sodium chloride in a small volume of dH2O, then
adjust the volume to 1 L
Calculate the mass in g of agarose required to make 200 mL of a
1.2% agarose gel. Record the answer to one decimal place. –
Answer 2.4
Calculate the mass in mg of kanamycin required to make 25 mL
of a 12 mg/mL solution of kanamycin. – Answer 300
Calculate the mass in g of sucrose required to make 125 mL of a
15.5% (w/v) sucrose solution. Record the correct amount to one
decimal place. – Answer 19.4
Calculate the mass in g of MgCl2 required to make 75 mL of a
20% (w/v) MgCl2 solution. Record the correct amount. –
Answer 15
BACE Exam Review Questions With
CoMPLETE SOLUTIONS
Which of the following is an example of a product easily found
in nature? – Answer Antibiotic
In a biotechnology lab, tap water is best used for – Answer
washing glassware
The order of amino acids on a polypeptide chain is encoded by –
Answer mRNA
How do DNA molecules vary from one species to another? –
Answer Number of genes and non-coding regions
What is agar? – Answer Solid media on which bacteria grow
What part of an amino acid differs from one to the other? –
Answer R group
Which of the following is an mRNA codon?
ATA
UAU
Tyrosine
All of the above – Answer UAU
How can colorless proteins be monitored during
electrophoresis? – Answer PAGE loading dye