To separate genomic DNA fragments by size, which of these laboratory methods is most useful?
A.
Filtration
B.
Spectrophotometry
C.
Titration
D.
Electrophoresis
The correct answer is:
D. Electrophoresis
Explanation:
Electrophoresis is a laboratory technique used to separate DNA fragments based on their size. The process involves applying an electric field to a gel matrix, which causes DNA fragments to migrate through the gel. Smaller fragments move faster and travel further through the gel, while larger fragments move more slowly. This separation allows for the analysis and identification of different DNA fragments.
Here’s why the other methods are less appropriate for separating DNA fragments by size:
- A. Filtration: This method is used to separate particles based on size but is not typically used for separating DNA fragments.
- B. Spectrophotometry: This technique measures the concentration of DNA or other molecules by assessing their absorbance of light but does not separate DNA fragments by size.
- C. Titration: This method is used to determine the concentration of a substance in a solution but is not used for separating DNA fragments.
Correct answer: D