Jon wanted to physically sterilize a plate but was worried that heat would melt it. What’s one method he could use that would protect and sterilize the fomite? (A) Pasteurization (B) Ionizing radiation (C) Filtration (D) Autoclave
The correct answer and explanation is:
The correct answer is (B) Ionizing radiation.
Ionizing radiation is an effective method of sterilizing objects without using heat, making it ideal for heat-sensitive materials like the plate Jon wants to sterilize. This method involves using high-energy radiation, such as gamma rays, X-rays, or electron beams, to kill or deactivate microorganisms on surfaces. The radiation penetrates the material, breaking down the DNA of microorganisms and rendering them incapable of reproduction, effectively sterilizing the fomite.
Unlike heat-based sterilization methods, ionizing radiation does not require high temperatures, so it is suitable for materials that may melt or degrade when exposed to heat. This is especially important for items made of plastic, certain polymers, or other sensitive materials that could be damaged by heat.
The other options are not as suitable for protecting the plate while sterilizing it:
- (A) Pasteurization involves heating an object to a temperature that is high enough to kill harmful microorganisms but low enough to avoid damaging the material. However, pasteurization still involves heat, which may not be appropriate for the plate Jon is worried about melting.
- (C) Filtration is a physical method that removes microorganisms by passing a fluid through a filter. While effective for liquids or gases, it cannot be used for solid objects like a plate, which requires surface sterilization.
- (D) Autoclave uses steam under high pressure and temperature to sterilize objects. While it is effective, the heat and pressure involved could potentially damage or melt the plate.
Ionizing radiation is a versatile and safe option for sterilizing heat-sensitive items without causing damage to the material.