MB ASCP CONNECT EXAM LATEST 2023-2024 ACTUAL
EXAM 200 QUESTIONS AND CORRECT ANSWERS WITH
RATIONALES|ALREADY GRADED A+
Purines and pyrimidines differ from each other in that:
a. Purines are found RNA; pyrimidines are found in DNA
b. There’s no difference between purines and pyrimidines
c. Pyrimidines have two rings; purines have one ring
d. Purines have two rings; pyrimidines have one ring – ANSWER- Purines have
two rings; pyrimidines have one ring
Rationale: TIP: Reciprocals of each other.
Purine, shorter word, longer rings.
Pyrimidine, longer word, shorter ring.
Which of the following molecular methodologies would be the best for detecting a
trinucleotide repeat disorder such as Huntington’s disease?
a. Heteroduplex analysis
b. Variable number tandem repeat analysis
c. Single strand conformation polymorphism analysis
d. Reverse-Transcriptase PCR – ANSWER- Variable number tandem repeat
analysis
Rationale: Locus Test C M PF1 PF2
A1 12/15 12 15/17 12/15
B2 13 13/13 13/15 14/15
C3 7/8 8 7 7/7
D4 9/11 9/9 10/11 11
E5 6 6/6 5/7 6
Based on the results above, which one of the two possible fathers is the two-month
old’s biological father?
a. Possible Father 1
b. Possible Father 2
c. Neither of the Possible Fathers – ANSWER- Neither
Sickle cell disease is an autosomal genetic disease due to a point mutation in the
beta-globin gene, where glutamic acid is substituted for valine at the sixth codon of
the gene, resulting in a faulty hemoglobin S (Hb S). Sickle cell disease is one of
many genetic diseases where a single gene controls the expression of many
phenotypic traits. The phenomenon where a single gene controls the expression of
many phenotypic traits is best referred to as:
a. Pleiotrophy
b. Polygenic inheritance
c. Epistasis
d. Epigenetics – ANSWER- Pleiotrophy
A technologist uses the spectrophotometer to quantify the amount of DNA
extracted from a blood specimen diluted 1:30. The absorbance reading at 260 nm
was found to be 2.545. If the absorbance at 280 nm gave a reading of 1.406, and if
the the DNA extract was re-suspended in 0.800 mL of EDTA solution, the DNA
yield is:
a. 3540 micrograms
b. 3450 micrograms
c. 3504 micrograms
d. 3054 micrograms – ANSWER- 3054 ug
Rationale: 260 reading x 50 ug/ml (DNA) x dilution factor x resupsension = DNA
Yield
260 x 50 x 30 x 0.8 = 3054
When sequencing HLA-DR what is targeted?
a. Exon 1 of the α subunit
b. Exon 2 of the α subunit
c. Exon 1 of the β subunit
d. Exon 2 of the β subunit – ANSWER- Exon 2 of the β subunit
All of the following are components of nucleic acids, EXCEPT:
a. Phosphate group
b. Sugar (ribose or deoxyribose)
c. Nitrogenous base (A, G, C, T)
d. Formamide – ANSWER- Formamide
This polymerase acts on DNA and produces Transfer RNA:
a. RNA Pol II
b. DNA Pol II
c. RNA Pol III
d. DNA Pol III – ANSWER- RNA pol III
In mantle cell lymphoma (MCL), this is the fusion gene created:
a. EWS-FLI1
b. CCND1-IGH
c. BCR-IGH
d. c-myc-EWS – ANSWER- CCND1-IGH
(Cyclin D1)
This enzyme is known for “unzipping” genes:
a. Alkaline Phosphatase
b. RNA polymerase
c. Apyrase
d. Helicase – ANSWER- Helicase
What translocation results in synovial sarcoma, a muscle and fibrous tissue cancer?
a. t(18;X)
b. t(15;17)
c. t(8;X)
d. t(9;12) – ANSWER- t(18;X)
Which of the following reagents will successfully decontaminate a bench top that
has been exposed to DNA amplification products?
a. 70% Ethanol
b. 5% Bleach
c. 100% Isopropanol
d. 10% Methanol – ANSWER- 5% Bleach
As a technologist working in a small clinical laboratory, a nasal swab specimen,
suspected to be colonized by a variety of upper respiratory viruses, came to your
laboratory. You are tasked with determining whether the specimen is colonized
with rhinovirus, coronavirus, and influenza. Which PCR method would best be
suitable for this task?
a. Nested PCR
b. Real-time PCR
c. NASBA
d. Multiplex PCR – ANSWER- Multiplex PCR
According to Chargaff’s rule of base pairing, adenine pairs with:
a. Cytosine
b. Threonine
c. Thymine
d. Thymidine – ANSWER- Tymine
The t(11;22) translocation is responsible for:
a. Follicular lymphoma
b. Papillary thyroid cancer
c. Synovial sarcoma
d. Ewing sarcoma – ANSWER- Ewing sarcoma
Rationale: TIP: Affects children and adults between the ages of 11 – 22
Replication forks, known as origins of DNA replication, are created by this
enzyme:
a. Ligase
b. Taq Polymerase
c. Primase
d. Helicase – ANSWER- Helicase
What enzyme is involved in LCR?