To separate genomic DNA fragments by size, which of these laboratory methods is most useful?
A.
Titration.
B.
Electrophoresis.
C.
Filtration.
D.
Spectrophotometry.
The correct answer is:
B. Electrophoresis
Explanation:
- Electrophoresis: This method is used to separate DNA fragments based on size. During electrophoresis, DNA fragments are placed in a gel and an electric current is applied. Smaller fragments move faster through the gel, allowing for separation by size.
- A. Titration: Titration is a technique used to determine the concentration of a substance in a solution, not for separating DNA fragments.
- C. Filtration: Filtration is a method used to separate particles from a liquid or gas based on size, but it is not typically used to separate DNA fragments.
- D. Spectrophotometry: Spectrophotometry is used to measure the absorbance of light by a sample, which can be used to quantify DNA concentration but not to separate DNA fragments by size.
Therefore, B. Electrophoresis is the most useful method for separating genomic DNA fragments by size.