{"id":112805,"date":"2023-08-15T20:38:23","date_gmt":"2023-08-15T20:38:23","guid":{"rendered":"https:\/\/learnexams.com\/blog\/?p=112805"},"modified":"2023-08-15T20:38:25","modified_gmt":"2023-08-15T20:38:25","slug":"mb-ascp-connect-exam-latest-2023-exam-100-questions-and-correct-answers-with-rationales100-verified-answersalready-graded-a","status":"publish","type":"post","link":"https:\/\/www.learnexams.com\/blog\/2023\/08\/15\/mb-ascp-connect-exam-latest-2023-exam-100-questions-and-correct-answers-with-rationales100-verified-answersalready-graded-a\/","title":{"rendered":"MB ASCP CONNECT EXAM LATEST 2023 EXAM 100 QUESTIONS AND CORRECT ANSWERS WITH RATIONALES(100% VERIFIED ANSWERS)|ALREADY GRADED A+"},"content":{"rendered":"\n

MB ASCP CONNECT EXAM LATEST 2023 EXAM 100
QUESTIONS AND CORRECT ANSWERS WITH
RATIONALES(100% VERIFIED ANSWERS)|ALREADY
GRADED A+<\/p>\n\n\n\n

    \n
  1. Which PCR method has the highest specificity? – ANSWER- Nested
    PCR<\/li>\n\n\n\n
  2. After an amplification procedure followed by gel electrophoresis, you
    took a photo of your gel. You notice consistent bands in all your gel
    lanes. However, you also see what appears to be a faint but noticeable
    band in your reagent blank lane. What is your best explanation for the
    reagent blank band? – ANSWER- There was obvious contamination in
    this PCR reaction<\/li>\n\n\n\n
  3. A technologist uses the spectrophotometer to quantify the amount of
    DNA extracted from a blood specimen diluted 1:30. The absorbance
    reading at 260 nm was found to be 2.545. The concentration of DNA in
    this extract is: – ANSWER- 3817.5 micrograms\/mL<\/li>\n\n\n\n
  4. This translocation creates a fusion protein between the Abl1 gene on
    one chromosome and the BCR gene on the other chromosome, resulting
    in chronic myelogenous leukemia (CML): – ANSWER- t(9;22)<\/li>\n\n\n\n
  5. The sugar in DNA is: – ANSWER- Deoxyribose<\/li>\n\n\n\n
  6. Primer dimers are due to what complimentary issue? – ANSWER- 3′
    to 3′ complementarity of primer pairs<\/li>\n\n\n\n
  7. What is the sequence recognized by poly (A) polymerase? –
    ANSWER- AAUAAA<\/li>\n\n\n\n
  8. In what part of a qPCR Amplification Plot do you take your
    measurement? – ANSWER- Exponential phase<\/li>\n\n\n\n
  9. In CML, what fusion protein is created? – ANSWER- BCR-Abl1<\/li>\n\n\n\n
  10. What assay amplifies the probe signal and the probe RNA
    concentration increases if the target to be detected is present? –
    ANSWER- Q\u03b2 replicase<\/li>\n\n\n\n
  11. Purines and pyrimidines differ from each other in that: – ANSWERPurines have two rings; pyrimidines have one ring<\/li>\n\n\n\n
  12. The mother of a 3 year old boy took him to the doctor’s office for
    concerns of an underlying genetic condition. A karyotype order was sent
    to the Molecular Diagnostic lab. Karyotype results show 47, XY,+21.
    Based on these results, the boy has: – ANSWER- Down Syndrome<\/li>\n\n\n\n
  13. In a retrovirus the RNA acts as: – ANSWER- Genome and mRNA<\/li>\n\n\n\n
  14. The enzyme that primes DNA synthesis is: – ANSWER- DNA
    primase<\/li>\n\n\n\n
  15. What are characteristics of the melt curve analysis? (hint: more than
    one answer) – ANSWER- At the melting point, the probe separates from
    the target strand and fluorescence rapidly decreases. The melting
    temperature of double stranded DNA depends on its base composition
    and length. All PCR products for a specific primer pair should have the
    same melting temperature.<\/li>\n\n\n\n
  16. What enzyme is crucial in the making RNA from DNA? –
    ANSWER- RNA Polymerase<\/li>\n\n\n\n
  17. How many polymorphisms can be found in the human genome? –
    ANSWER- Approximately 10 million<\/li>\n\n\n\n
  18. A DNA specimen was sent to your Molecular Diagnostics
    Laboratory for DNA sequencing, in order to determine whether patient<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

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