{"id":202087,"date":"2025-03-17T07:24:52","date_gmt":"2025-03-17T07:24:52","guid":{"rendered":"https:\/\/learnexams.com\/blog\/?p=202087"},"modified":"2025-03-17T07:24:54","modified_gmt":"2025-03-17T07:24:54","slug":"which-objective-focuses-closest-to-the-slide","status":"publish","type":"post","link":"https:\/\/www.learnexams.com\/blog\/2025\/03\/17\/which-objective-focuses-closest-to-the-slide\/","title":{"rendered":"\u00a0Which objective focuses closest to the slide"},"content":{"rendered":"\n<p>\u00a0Which objective focuses closest to the slide?<\/p>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-ast-global-color-6-color\"><strong>The correct answer and explanation is :<\/strong><\/mark><\/p>\n\n\n\n<p>The objective that focuses closest to the slide is the <strong>oil immersion objective<\/strong> (usually <strong>100x<\/strong> magnification).<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">Explanation:<\/h3>\n\n\n\n<p>A compound light microscope typically has multiple objective lenses:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Scanning Objective (4x):<\/strong> Provides the widest field of view and lowest magnification, used for initial observation.<\/li>\n\n\n\n<li><strong>Low-Power Objective (10x):<\/strong> Offers a closer view than 4x, commonly used for observing larger details.<\/li>\n\n\n\n<li><strong>High-Power Objective (40x):<\/strong> Provides greater magnification for detailed examination of specimen structures.<\/li>\n\n\n\n<li><strong>Oil Immersion Objective (100x):<\/strong> Requires a drop of immersion oil to eliminate air gaps, increasing resolution and focusing the closest to the slide.<\/li>\n<\/ul>\n\n\n\n<p>The <strong>oil immersion objective<\/strong> focuses closest to the slide because:<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li><strong>Shorter Working Distance:<\/strong> This is the space between the objective lens and the slide when the specimen is in focus. The 100x objective has an extremely short working distance, often less than <strong>0.2 mm<\/strong>.<\/li>\n\n\n\n<li><strong>Use of Immersion Oil:<\/strong> The oil immersion technique reduces light refraction by matching the refractive index of glass, ensuring clearer and sharper images. Without the oil, light bends away, reducing resolution.<\/li>\n\n\n\n<li><strong>Maximum Magnification and Detail:<\/strong> The 100x objective allows for the highest possible magnification in a standard light microscope, making it ideal for observing bacteria, blood cells, and fine cellular structures.<\/li>\n<\/ol>\n\n\n\n<p>Since the 100x oil immersion objective is designed to be as close as possible to the specimen while maintaining clarity, it is the correct answer to the question.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u00a0Which objective focuses closest to the slide? The correct answer and explanation is : The objective that focuses closest to the slide is the oil immersion objective (usually 100x magnification). Explanation: A compound light microscope typically has multiple objective lenses: The oil immersion objective focuses closest to the slide because: Since the 100x oil immersion [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[25],"tags":[],"class_list":["post-202087","post","type-post","status-publish","format-standard","hentry","category-exams-certification"],"_links":{"self":[{"href":"https:\/\/www.learnexams.com\/blog\/wp-json\/wp\/v2\/posts\/202087","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.learnexams.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.learnexams.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.learnexams.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.learnexams.com\/blog\/wp-json\/wp\/v2\/comments?post=202087"}],"version-history":[{"count":0,"href":"https:\/\/www.learnexams.com\/blog\/wp-json\/wp\/v2\/posts\/202087\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.learnexams.com\/blog\/wp-json\/wp\/v2\/media?parent=202087"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.learnexams.com\/blog\/wp-json\/wp\/v2\/categories?post=202087"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.learnexams.com\/blog\/wp-json\/wp\/v2\/tags?post=202087"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}