{"id":238502,"date":"2025-06-18T07:36:34","date_gmt":"2025-06-18T07:36:34","guid":{"rendered":"https:\/\/learnexams.com\/blog\/?p=238502"},"modified":"2025-06-18T07:36:37","modified_gmt":"2025-06-18T07:36:37","slug":"remembering-that-dna-polymerases-can-only-add-nucleotides-to-the-3-end-of-dna-design-forward-primer-and-reverse-primer-each-10-bases-long-amplify-target-sequence-of-the-dna-that-is-at-least-100","status":"publish","type":"post","link":"https:\/\/www.learnexams.com\/blog\/2025\/06\/18\/remembering-that-dna-polymerases-can-only-add-nucleotides-to-the-3-end-of-dna-design-forward-primer-and-reverse-primer-each-10-bases-long-amplify-target-sequence-of-the-dna-that-is-at-least-100\/","title":{"rendered":"Remembering that DNA polymerases can only add nucleotides to the 3′ end of DNA design = forward primer and reverse primer; each 10 bases long; amplify target sequence of the DNA that is at least 100 bp long: Write the sequence of the primers below; with their 3′ and 5′ ends indicated."},"content":{"rendered":"\n

Remembering that DNA polymerases can only add nucleotides to the 3′ end of DNA design = forward primer and reverse primer; each 10 bases long; amplify target sequence of the DNA that is at least 100 bp long: Write the sequence of the primers below; with their 3′ and 5′ ends indicated. Also indicate on the sequence above which strand they are complementary to (will anneal to). Forward primer sequence: 5′ AGGAAGGTGG 3′ Reverse primer sequence: 5′ CTTCTGCAAG 3<\/p>\n\n\n\n

The Correct Answer and Explanation is:<\/mark><\/strong><\/p>\n\n\n\n

To design forward and reverse primers correctly for amplifying a DNA sequence, it is essential to understand both the directionality of DNA strands and the role of DNA polymerase. DNA polymerase can only synthesize DNA in the 5′ to 3′ direction<\/strong>, meaning primers must bind to the 3′ ends of the template strands<\/strong> and point toward each other across the target region.<\/p>\n\n\n\n

Given:<\/p>\n\n\n\n