ACS BIOCHEMISTRY EXAM QUESTIONS AND CORRECT SOLUTIONS

ACS BIOCHEMISTRY EXAM QUESTIONS AND CORRECT SOLUTIONS
Question :Henderson-Hasselbach Equation
CORRECT ANSWER:pH = pKa + log ([A-] / [HA])
Question :FMOC Chemical Synthesis
CORRECT ANSWER:Used in synthesis of a growing
amino acid chain to a polystyrene bead. FMOC is used as
a protecting group on the N-terminus.
Question :Salting Out (Purification)
CORRECT ANSWER:Changes soluble protein to solid
precipitate. Protein precipitates when the charges on the
protein match the charges in the solution.
Question :Size-Exclusion Chromatography
CORRECT ANSWER:Separates sample based on size
with smaller molecules eluting later.

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