BIO 113 LAB FINAL, DR. METZLER BALL STATE UNIVERSITY

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BIO 113 LAB FINAL, DR. METZLER BALL STATE UNIVERSITY

EXAM QUESTIONS

Actual Qs and Ans - Expert-Verified Explanation -Guaranteed passing score -100 Questions and Answers

-Format: Multiple-choice / Flashcard

Question 1: Urea

Answer:

• end product of amino aid metabolism in humans
• urea broth light orange in neutral conditions
• urea broth pink basic ph

Question 2: Endospore

Answer:

• like armor for bacteria
• make bacteria resistant to harsh conditions

Question 3: Dry heat

Answer:

• moderate to high temperatures
• dehydration, alters protein - incineration
• less efficient than moist heat

Question 4: Spirilli/spirochete

Answer:

corkscrew

Question 5: Methyl red

Answer:

an acid-base indicator named after its colour change from red to yellow over a pH range of 4.8 to 6.0

Question 6: Degerming

Answer:

reduce microbes on skin or tissue through mechanical means & mild chemicals

- ex: hand washing

Question 7: Obligate aerobes

Answer:

require oxygen

Question 8: Antibiotic

Answer:

any substance secreted by microbe & inhibits another microbe

Question 9: fermentative catabolism

Answer:

Doesn't require oxygen but can occur in its presence.

• occurs if growth happens in anaerobic environment of O/F media

Question 10: Starch Agar

Answer:

• iodine bind to starch create blue/black compound
• areas w/ starch hydrolyzed turn tan
• if tan only where bacteria is hydrolyzed within cells
• if tan areas extend perimeters of bacteria, amylase degrade starch outside cells

Question 11: O/F media

Answer:

• oxidative fermentative media
• high concentration glucose
• low concentration of peptone (partially digested proteins)
• ph indicator bromthymol blue
• acidic=yellow, green=neutral, blue=alkaline
• fermentation byproduct is acid (turn media yellow)

- protein metabolism: alkaline ammonia produced (media blue)

Question 12: Microaerophiles

Answer:

can use oxygen only when it is present at levels reduced from that in air

Question 13: streak plate

Answer:

designed to isolate singly colonies on agar

Question 14: Differential media

Answer:

make it easy to distinguish colonies of different microbes

Question 15: Hydrogen peroxide

Answer:

oxidizing chemical produced as byproduct of other metabolic reactions

Question 16: colony of bacteria

Answer:

A cluster of cells which arises from a single bacterium by asexual reproduction

Question 17: Epidemiology

Answer:

Branch of medical science concerned with the incidence, distribution, and control of diseases that affect large numbers of people.

Question 18: Plasmid

Answer:

small ring of DNA carries extra genes separate from those of the bacterial chromosome

Question 19: TSI agar

Answer:

• triple sugar iron agar
• contain phenol red, lactose, sucrose, small amount of glucose, amino acid, & iron
• yellow= acidic(fermentation of sugars), neutral/alkaline= red
• ferment lactose or sucrose= bottom & slant yellow
• ferment glucose only= yellow bottom, red slant
• desulfurization (H2S produced)= black

• gas produced= bubbles, bottom pushed up from glass

Question 20: Controlling growth using heat

Answer:

as temperature rises bacterial growth decreases

Question 21: Type O blood

Answer:

• universal donor
• antibodies anti-A and anti-B
• receive from O

Question 22: Blood Agar

Answer:

• differential

Question 23: Moist heat

Answer:

• transfer thermal energy well
• pasteurization, boiling, autoclaving

Question 24: Sanitization

Answer:

removal of pathogens from objects to meet public health standards

Ex: cleaning restrooms

Question 25: Gram-positive bacteria

Answer:

• stain purple

-thick cell wall -peptidoglycan + lipoteichoic acid

Question 26: Mutations caused by UV radiation

Answer:

less colonies created as more exposure to radiation