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1. What is the main difference between BSL-2 and BSL-3
in terms of laboratory design and practices?
a) BSL-3 requires negative air pressure and HEPA
filtration of exhaust air.
b) BSL-3 requires biosafety cabinets and personal
protective equipment for all procedures.
c) BSL-3 requires a separate changing room and shower
facility for personnel.
d) BSL-3 requires a double-door entry and an anteroom for
decontamination.
*Answer: a) BSL-3 requires negative air pressure and
HEPA filtration of exhaust air. Rationale: This is the main
feature that distinguishes BSL-3 from BSL-2, as it prevents
the escape of potentially infectious aerosols from the
laboratory.*
2. Which of the following agents is not typically handled in
a BSL-3 laboratory?
a) Mycobacterium tuberculosis
b) Bacillus anthracis
c) Ebola virus
d) Francisella tularensis
*Answer: c) Ebola virus. Rationale: Ebola virus is
classified as a Risk Group 4 agent, which requires BSL-4Â
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