FREE AND STUDY GAMES ABOUT MICO LAB QUIZ 2 EXAM

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FREE AND STUDY GAMES ABOUT MICO LAB QUIZ 2 EXAM

QUESTIONS

Actual Qs and Ans Expert-Verified Explanation

This Exam contains:

-Guarantee passing score -32 Questions and Answers -format set of multiple-choice -Expert-Verified Explanation Question 1: If you saw eukaryotic cells on your gum line, they were most likely your own epithelial cells. Are you gram + or harm -. What is it about your cell structure that would allow you to predict this result

Answer:

Cells act like gram - cells. Epithelial cells have a plasma membrane and no cell wall

Question 2: What does the steam do durning a endospore stain

Answer:

Drives green stain into spores

Question 3: What bacteria was used for the capsule stain

Answer:

Klebsiella pneumoniae

Question 4: Why a no heat fixation step durning a capsule stain

Answer:

Causes cells to shrink, leaves and artificial white layer around cell, mix cells with serum or albumin--makes cells adhere to slide

Question 5: Why are capsules important

Answer:

Increase virulence by resisting phagocytosis

Question 6: Gram stain

Answer:

1st test run on a specimen brought into a lab

Question 7: Acidic stain

Answer:

Background (Congo red)

Question 8: Failure to apply the safranin in gram stain

Answer:

No counter stain. + will be purple - will be colorless

Question 9: Failure to apply the decolorizer in gram stain

Answer:

Decolorizer- removes primary stain in gram -. No decolorizer means the crystal violet retained by gram

• Both + and - will be purple

Question 10: Failure to add the iodine in gram stain

Answer:

Crystal violet will wash off of gram +. Both + and - will be pink

Question 11: Capsule is colorless

Answer:

White clearing around stain

Question 12: Reversal of crystal violet and safranin stains in gram stain

Answer:

Safranin washed out of gram - cells. Counter stain is darker than primary stain so both gram + and - will be purple

Question 13: How is a capsule stain different

Answer:

Use a negative staining process, acidic stain, capsule is colorless

Question 14: Shape of the spore

Answer:

Spherical, oval

Question 15: Why gram + purple

Answer:

Thick peptidoglycan cell wall, holds tight to crystal violet after iodine sets the stain

Question 16: Basic stain

Answer:

Cell (manevals stain)

Question 17: Why does this excerise call for 5 day old culture of Bacillus

Answer:

• day old culture is dying off, lacking in nutrients, which triggers sporulation

Question 18: Where can the location of the spores be

Answer:

Center, terminal, subterminal

Question 19: What is a gram stain used for

Answer:

To distinguish between a gram + cell and a gram - cell Question 20: What happens for the cells to stain pink durning an endospore stain

Answer:

Green washes out of cell durning decolonization(water), and cells take on counter stain

Question 21: What is the most common differential stain

Answer:

Gram stain

Question 22: Endospores

Answer:

Dormant form of a bacterium, allows it to survive poor environment, cell will form spores when

requirements for life are unavailable ie: nutrients, oxygen, water

Question 23: Why gram - pink

Answer:

Thin cell wall and LPS outer membrane, cell takes in color of secondary stain-- safranin

Question 24: What does a endospore stain do for us

Answer:

Differential stain used to detect endospores

Question 25: What bacteria was used for the gram - gram stain

Answer:

Escherichia coli

Question 26: What bacteria was used for the gram + gram stain

Answer:

Staphylococcus epidermidis Question 27: Theoretically could you use any color for your primary and counter stain as long as their not the same color

Answer:

Yes it would be best if the primary stain was darker; counter stain lighter

Question 28: What does the endospore staining process help

Answer:

Helps us to identify bacterial species because location and shape of spores is unique