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FREE AND STUDY GAMES ABOUT MICRO LAB EXERCISE 5
EXAM QUESTIONS
Actual Qs and Ans Expert-Verified Explanation
This Exam contains:
-Guarantee passing score -16 Questions and Answers -format set of multiple-choice -Expert-Verified Explanation
Question 1: Of what value is a simple stain?
Answer:
To observe morphology and arrangement.
Question 2: And heat-fixing, what would happen if too much heat were applied?
Answer:
The slide could break. Cells could rupture. The cells could burn and become distorted.Question 3: What stains can be used to determine cell morphology, size, and arrangement?
Answer:
Simple Stains Question 4: The dry smear is passed through a Bunsen burner flame several times to ________ the bacteria. This may not kill all of the bacteria.
Answer:
Heat fix
Question 5: If the chromophore is a positive ion, the stain is considered a?
Answer:
Basic Stain
Question 6: How long should you allow the loop to cool for?
Answer:
About 30 seconds
Question 7: A simple stain that stains the bacteria is a?
Answer:
Direct Stain Question 8: A thin film of bacterial cells placed on a slide to stain is called a?
Answer:
Smear Question 9: Methylene blue can be prepared as a basic stain or acidic stain. How would the pH of the stain affect the staining of bacteria?
Answer:
The higher the pH the more basic it is. It has a positive charge.Question 10: Alternatively, the dry smear can be placed on a ______ slide warmer for _____ minutes.
Answer:
60°C for 5 minutes Question 11: A simple stain that stains the background but leaves the bacteria I stained is a?
Answer:
Negative Stain Question 12: What must be done to the smear to kill the bacteria? This action also preserves microbes with minimal shrinkage or distortion when stained.
Answer:
It must be fixed
Question 13: What is the purpose of heat-fixing the smear?
Answer:
If you don't, when you do the wash steps, the specimen will wash off.
Question 14: Direct stain is also referred to as?
Answer:
A basic stain.
Question 15: Staining procedures that use only one stain are called?
Answer:
Simple Stains
Question 16: If the chromophore is a negative ion, the stain is considered a?
Answer:
Acidic Stain