FREE AND STUDY GAMES ABOUT MICROBIOLOGY FINAL

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FREE AND STUDY GAMES ABOUT MICROBIOLOGY FINAL

EXAM QUESTIONS

Actual Qs and Ans Expert-Verified Explanation

This Exam contains:

-Guarantee passing score -86 Questions and Answers -format set of multiple-choice -Expert-Verified Explanation

Question 1: What shape are Diplococci & Diplobacilli bacteria?

Answer:

Pairs of cells Question 2: A bacterial plate count form a urine culture of 1000 - 10000 bacteria/mL = ?

Answer:

Probably contamination of sample

Question 3: How would a Gram Stain be used with fungi?

Answer:

Yeast stain gram positive and can be distinguished from bacteria, which are smaller then yeast.

Question 4: Where does yeast Cryptococcus cause serious infection?

Answer:

It can cause serious infections in the lungs. It is also thought to cause meningitis.

Question 5: What are the 3 steps in PCR?

Answer:

• Separation of the strands,
• Annealing the primer to the template,
• Synthesis of the new strand.

Question 6: Define what a titer is.

Answer:

A serial dilution of serum is made & mixed with a fixed amount of antigen and agglutination or precipitation of the test tube is looked for. The highest dilution showing a positive reaction is the titer.

Question 7: Briefly, what is PCR?

Answer:

Polymerase Chain Reaction. It is a technique that allows many copies of DNA to be made.

Question 8: What is 16S rDNA?

Answer:

16S rDNA is one of the 3 RNA molecules associated with the ribosomes of prokaryotes. It can be used as a gene to be copied

Question 9: Describe Alpha Hemolysis.

Answer:

Ex. Streptococci able to lyse erythrocytes but only partially break down the heme pigment develop a green halo around each colony.

Question 10: What is the catalase test for and what reagent does it use?

Answer:

The purpose of the catalase test is to differentiate staphylococci from streptococci. This is possible because streptococci does not produce catalase and all staphylococci produce catalase. Hydrogen Peroxide (H202) is used.

Question 11: What is the name of the acid-fast stain and what is it used for?

Answer:

Ziehl-Neelsen procedure. Acid fast organisms possess a cell wall rich in lipids, complex lipids and waxes. Primary stain is driven into the cell using heat, by steaming. Acid fast cells appear red.Non-acid-fast cells are counterstained and appear blue.

Question 12: What disease does Clostridium Botulinum casue?

Answer:

Botulism

Question 13: Is a blood agar plate selective or differential (both or neither)?

Answer:

Differential

Question 14: How would an India Ink Preparation be used with fungi?

Answer:

Preparation of spinal fluid is made when Cryptococcus is suspected to be causing meningitis.Cryptococcus can have a large capsule which in an India Ink Preparation will show as a clear area surrounding the yeast cell.

Question 15: What is Selective Media?

Answer:

Specific for the isolation or cultivation of a specific organism.Question 16: Why are sometimes molecular techniques used to identify pathogens instead of traditional methods?

Answer:

B/C many pathogens grow poorly on solid medium while others grow only in liquid culture, making identification through traditional techniques difficult or impossible. With the aid of molecular methods, however, these limitation can be overcome.

Question 17: What is the scotch tape test?

Answer:

When pinworm infection diagnosis is found using scotch tape. It is put against the perianal folds and the pinworm eggs stick to tape.Question 18: A bacterial plate count from a urine culture of >100000 bacteria/mL = ?

Answer:

Establishes the presence of a UTI

Question 19: How would an individual get a Trichinella infection?

Answer:

By eating raw or undercooked pork and wild game infected with the larvae of a parasitic worm.

Question 20: What is the Coagulase test for?

Answer:

It is used to differentiate Staphylococcus Aureus from other forms of Staphylococci. Staphylococcus produces Coagulase and other forms of staphylococci do not.Question 21: In what host does the sexual reproductive cycle of Toxoplasma gondii occur?

Answer:

CATS Question 22: In which host does the sexual reproductive cycle of plasmodia take place?

Answer:

In mosquitoes.

Question 23: What stain shows a capsule?

Answer:

A counterstain such as Gin Stain. Methylene blue is also often used as the counterstain. When observed under the microscope such a preparation shows halos or clear rings around pale blue bacterial cells against a dark background.Question 24: What is the infective stage of Trichomonas and how is it diagnosed?

Answer:

Sexual transmission of trophozoite (NO CYST STAGE). Genital secretions for a wet mount to look for moving trophozoite.

Question 25: Give the insect vector for Trypanosoma brucei.

Answer:

Tsetse Fly (Glossina) Question 26: What 2 parasites use direct skin penetration as the way to enter a new host?

Answer:

Schistosoma (Blood Fluke), Necator Americanus (Hookworm)