FREE SCIENCE AND STUDY GAMES ABOUT CLINICAL

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FREE SCIENCE AND STUDY GAMES ABOUT CLINICAL

CHEM 1 EXAM QUESTIONS

Actual Qs and Ans Expert-Verified Explanation

This Exam contains:

-Guarantee passing score -69 Questions and Answers -format set of multiple-choice -Expert-Verified Explanation

Question 1: Most atomic absorption spectrophotometers

incorporate a beam chopper and a tuned amplifier. The purpose of these components is to avoid errors that would be caused by

Answer:

Measurement of light emitted by the analyte

Question 2: The measurement of oxygen in blood by

means of a PO2 electrode involves which of the following?

Answer:

Direct relationship between amount of oxygen in the sample and amount of current flowing in the measuring system

Question 3: Which of the following may be associated

with bioluminescence?

Answer:

Light emission produced due to enzymatic oxidation of a substrate

Question 4: Which parameter is used in mass

spectrometry to identify a compound?

Answer:

Ion mass-to-charge ratio Question 5: Which of the following is not descriptive of a photomultiplier tube?

• Emits electrons proportionally to

initial light absorbed

• Must be shielded from stray light
• Cannot be used with a chopper
• Amplifies the initial signal received

Answer:

Cannot be used with a chopper

Question 6: To calibrate the pH electrode in a pH/

blood gas analyzer, it is necessary that

Answer:

Two buffer solutions of known pH be used

Question 7: Which of the following chromatography

systems may be described as having a stationary phase that is liquid absorbed on particles packed in a column and a liquid moving phase that is pumped through a column?

Answer:

High-performance liquid

Question 8: Because of similar electrophoretic mobilities,

several hemoglobins cannot be differentiated on cellulose acetate medium.Electrophoresis of hemoglobins at pH 6.2 on agar gel may be useful in differentiating which hemoglobins?

Answer:

C from A2

Question 9: Which term describes a congenital

disorder that is characterized by a split in the albumin band when serum is subjected to electrophoresis?

Answer:

Bisalbuminemia

Question 10: When using EMIT, the enzyme is coupled

to

Answer:

Antigen

Question 11: What is the purpose of using ampholytes

in isoelectric focusing?

Answer:

Establish a pH gradient in the gel

Question 12: Which disorder is not associated with an

elevated protein level in cerebrospinal fluid?

Answer:

Hyperthyroidism

Question 13: When quantifying glucose using an

amperometric glucose electrode system, which of the following is not a component of the system?

Answer:

Hexokinase reacts with glucose

Question 14: When performing electrophoresis at pH

8.6, which hemoglobin molecule migrates the fastest on cellulose acetate toward the anode?

Answer:

A1

Question 15: Which of the following is an electrophoretic

technique employing a pH gradient that separates molecules with similar isoelectric points?

Answer:

Isoelectric focusing

Question 16: Which of the following is not associated

with silver stains?

• Reactive to nanogram concentrations

of proteins

• Polypeptides stain a variety of colors
• Not as sensitive as Coomassie brilliant

blue

• Preconcentration of CSF not

necessary

Answer:

Not as sensitive as Coomassie brilliant blue

Question 17: Proteins, carbohydrates, and lipids are the

three major biochemical compounds of human metabolism. What is the element that distinguishes proteins from carbohydrate and lipid compounds?

Answer:

Nitrogen

Question 18: Hemoglobin S is an abnormal hemoglobin

that is characterized by a substitution of which amino acid?

Answer:

Valine for glutamic acid in position 6 on the beta chain