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FREE SCIENCE AND STUDY GAMES ABOUT MICRO-LAB
EXAM QUESTIONS
Actual Qs and Ans Expert-Verified Explanation
This Exam contains:
-Guarantee passing score -13 Questions and Answers -format set of multiple-choice -Expert-Verified Explanation
Question 1: What is the use of Petri plates in microbiology?
Answer:
They provide a large surface area for solid culture media in an easily stored container Question 2: Why is the swab first moistened in sterile water before sampling a dry surface?
Answer:
the swab must be moist to adequately pick up microbes.Question 3: Did all the organisms living in or on the environments sampled grow on your nutrient agar? Why or why not?
Answer:
No because we didnt satisfy all the nutritional and environmental requirements
Question 4: What is human body temperature in degrees celcius?
Answer:
37 degrees celcius
Question 5: Why is condensation on the agar undesirable?
Answer:
Bacteria will be in a liquid suspension instead of forming discrete colonies and it will spread the microbes on the surface Question 6: Why is agar preferable to gelatin as a solidifying agent in culture media?
Answer:
- It liquefies at 100 degrees Celsius and stays in a liquid state until cooled to 40 degrees Celsius.
- Once hardened, it stays solid up to 100 degrees Celsius.
- Few microbes can metabolize the agar, so it remains solid.
Question 7: How could you tell whether the turbidity in your nutrient broth was from a mixture of different microbes or from the growth of only one kind of microbe?
Answer:
By sub-culturing the nutrient broth onto the surface of a nutrient agar plate. The plate will create discrete colonies & if they differ u can tell.Question 8: Why is it necessary to keep the flask at an angle through this procedure?
Answer:
To minimize contamination from the air
Question 9: What quantity of agar will you need to add?
Answer:
1.28 g
Question 10: To what temperature will it have to be heated?
Answer:
100 degrees celcius
Question 11: What are bacteria using for nutrients in nutrient agar?
Answer:
Peptone and beef extract
Question 12: What is the purpose of the agar?
Answer:
The agar is the solidifying agent.
Question 13: What effect does the agar have on the culture medium?
Answer:
It solidifies the medium