1. Autoclaving isthe best method of sterilization because:
â It is bacteriostatic → bactericidal
â The pressure makes the organism more sensitive to heat
â It kills both the spores and vegetative cells
â The high temperature oxidizes bacteria
*Uses moist heatsignificantly affects coagulation of proteins (Ex. Pressure Cooker)
2. Alpha hemolytic streptococci are the most abundant:
â Wound contaminating organisms → combination of aerobic and anaerobic
â Skin contaminants → Staphylococcus epidermidis, Enterococcus, Viridans strep
â Normal throat bacteria → Streptococcus viridans
â Normal stool organisms → enterococci, enterobacter, Klebsiella
3. Based on their oxygen requirement, Clostridium is classified as:
â Obligate aerobe - Pseudomonas
â Microaerophile → Campylobacter
â Facultative anaerobe → most pathogenic organisms; Enterobacteriaceae
â Anaerobe → GRAM (+), SPORE FORMING
4. The presence of pili increases bacterial virulence by:
â Increasing attachment to host cells → sex pili involved in exchange of genetic
material
â Preventing phagocytosis → capsule
â Blocking surface antibodies→ biofilm
â Producing bacterial toxins→ endotoxin and exotoxin present in gram (+) and (-)
organisms
5. Toxic shock syndrome in menstruating women is caused by?
â Staphylococcus aureus → TSS; fever, rash, desquamation
â Staphylococcus epidermidis
â Streptococcus pyogenes
â Streptococcus agalactiae
6. A nonhemolytic, catalase positive, coagulase negative, Gram-positive cocci commonly
associated with urinary tract infection is most likely
â Staphylococcus epidermidis → novobiocin sensitive
â Staphylococcus saprophyticus → Novobiocin resistant
â Streptococcus pyogenes → catalase negative; Bacitracin sensitive
â Streptococcus agalactiae → catalase negative