MICROBIOLOGY VIVA 100 QUESTIONS

MICROBIOLOGY VIVA 100 QUESTIONS
What microbial factors lead to failure of endodontic treatment? - answer -presence of tough resistance
species of bacteria like e. Faecalis to ABs
-lack of contact time with microbial agents, limited penetration into endodontic biofilm
-Endontic biofilm resistance to ABs
Are spore forming bacteria normally found in mouth? - answer
How do we acquire the oral flora? - answer
What are the phases of bacterial growth? - answer 4 phases: lag, exponential, stationary, death
How do you take a specimen for microscopy/cultural/sensitivity MCS tests - answer Swab
What are the side effects of
-amoxicillin
-metronidazole
-tetracyclines - answer Amoxicillin: stomach upset, headache, skin rash
Metronidazole: stomach upset, headache, nausea
Tetracyclines:nausea, vomiting , diarrhea
What factors make dentoalveolar infections more likely to spread to distance sites - answer - Multiple
severe dental infections happening in the mouth left untreated or no drainage
-immunocompromised
-favorable location (maxillary molar and the sinus proximity )
-continued presence of source of infection
What precautions to take with patient with
-active TB
MICROBIOLOGY VIVA 100 QUESTIONS
-active CJD - answer
How is Hep C transmitted - answer Contact with blood. Tattoo piercing, sharing of needles, blood
transfusions, occupational incidents
Why is there no vaccine for Hep D? - answer -has multiple genotypes and variants
-mutates continually, evasion mechanisms in the innate and adaptive immune systems
Side effects of anti-retro viral tx or prophylaxis for HIV - answer Headache, nausea, vomiting, rash
How does an ELISA test work - answer Enzyme linked immunosorbent assay rely on specific antibodies to
bind the target antigen and a detection system to indicate the present and quantity of the antigen
binding. The microplate is coated with high affinity antibodies to increase sensitivity and precision of the
assay
How are bacteria identified in the lab - answer Bacterial cultures
How might the lab identity C. Albicans, is it normal to find fungus in mouth - answer -Take a blood
sample and culture it
-take the candida antibody test to detect systemic candidiasis by detecting antibodies that form
immunity against it
It is part of the normal microbiota of the mouth, commensal flora
How to test a lesion for viruses - answer -cotton swab the lesion and place in culture dish to grow
Why do antibiotics sometimes not work? - answer -AB resistance
-incorrect choice of antibiotics, wrong mechanism of action to target the desired pathogen
-incorrect dosage and timing of intake

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