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1. Describe the concept of gene editing and its potential applications in
biomedicine.
Answer: Gene editing is a technique that allows researchers to modify an
organism's DNA, including the human genome, with precision. It has the
potential to treat genetic diseases, develop new therapies, and enhance
crop production. One widely used gene editing tool is CRISPR-Cas9,
which enables scientists to edit DNA by cutting and modifying specific
genes.
Rationale: Gene editing is a rapidly advancing field of biomedicine that
has the potential to revolutionize medicine. Understanding its principles
and applications is crucial for health science students to comprehend its
impact on human health.
2. Explain the process of polymerase chain reaction (PCR) and how it is
utilized in biomedical research.
Answer: PCR is a technique used to amplify a specific segment of DNA.
It involves multiple cycles of heating and cooling to replicate the DNA
segment of interest. PCR plays a significant role in biomedicine, including
gene sequencing, diagnosis of genetic diseases, and forensic analysis.
Rationale: PCR is a fundamental tool in molecular biology and has
numerous applications in biomedicine. Knowledge of PCR techniques is
essential for health science students to understand various diagnostic and
research processes.
3. Discuss the principles of next-generation sequencing (NGS) and its
significance in genomic research.
Answer: Next-generation sequencing refers to cutting-edge DNA
sequencing technologies that allow the rapid and cost-effective sequencing
of entire genomes. NGS has revolutionized genomic research by enablingÂ
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