PORTAGE LEARNING BIOD 171 MICROBIOLOGY LAB NOTE BOOK LAB 1-9.

PORTAGE LEARNING

BIO 171

MICRO-BILOGY

Lab Notebook

 Lab Notebook Bookmarks

 Lab 1 Notebook

Lab 2 Notebook

Lab 3 Notebook

Lab 4 Notebook

Lab 5 Notebook

Lab 6 Notebook

Lab 7 Notebook

Lab 8 Notebook

Lab 9 Notebook

Lab 1 Notebook

Title: KD01 Introduction to medical microbiology & lab notebook procedures

Course Objectives:

Cultivation of samples: growth conditions and equipment used

Identification of samples: biochemical assays & tests available

Evaluation ofsamples: microscopy- visualization and key characteristics ofspecimens

Basic Equipment:

Cleaning: Autoclave 125°C- uses heat, pressure, & steam to sterilize; autoclave will take

Minutes to sterilize & hot dry air which will take hours; before

opening make sure it is depressurized and will have very hot air.

Growing (bacteria/pathogens) – Fixed incubator 37°C is airtight, shelving for petri dishes

- Shaker incubator 37°C shakes culture & rotates to aerateliquid nutrient broth

Visualizing: microscopy

Storing: refrigerator @ 4°C to stop the growth and helps preserve samples & keeps long term

Lab Safety

1. Never eat or drink in the lab: contamination risks

2. Use PPE (personal protection equipment): gloves- latex, nitrile (purple, blue, green),thermal,

or cold gloves (liquid nitrogen): eyewear: lab coat- chemical spills

3. Never leave lab while wearing PPE: including bathroom, hallway, or cafeteria

Outline of how to set up Notebook

Objective: To establish an organized template for keeping experimentalrecords, procedures,

and results.

Procedure:

1. This is where each step of a given protocol is recorded

2. Be sure to clearly indicate any deviations that may occur during the experiment- put

them in red

Notes: Additional (helpful) information placed here

Results: A summary of the final outcome of the experiment should be described here

Lab 2 Notebook

Title: KD02 Introduction to Microscopy

Objective: To learn basic parts of microscope and how to view a sample

Procedure:

1. Review the parts of microscope

2. Load sample to be viewed

3. Choose magnification

4. Adjust microscope so the sample is clear

Notes:

Parts of microscope:

1. eye pieces (ocular lens): can be pulled apart; should be able to use both eyes and see one

circle if not adjust

2. arm/neck: if moving this is what you will grab with one hand and hand under base

3. Objective lenses: this provides the magnification of the sample; shortest has the least

magnification (4x, 10x, 40x,100x)

4. Stage: the flat surface that you place your sample; holds the sample via a clamp (squeeze

together to place sample and release); stage clips raise up and lay on the glass coverslip

5. Focus knobs: located on the side of the arm/neck; Outside ring is the coarse adjustment –

makes large steps in focus; Inner ring is the fine focus- you can see the specimen but need

more detail