FREE AND STUDY GAMES ABOUT BIOCHEM TEST 1 EXAM

PDF Download

FREE AND STUDY GAMES ABOUT BIOCHEM TEST 1 EXAM

QUESTIONS

Actual Qs and Ans Expert-Verified Explanation

This Exam contains:

-Guarantee passing score -369 Questions and Answers -format set of multiple-choice -Expert-Verified Explanation

Question 1: biochemistry is _______ in our daily life

Answer:

indispensible (absolutely necessary) - ex: eating lunch

Question 2: ionization of amino acids - At neutral pH, the carboxyl group is______ but the amino group is______. The net charge is zero; such ions are called______.

Answer:

deprotonated; protonated; Zwitterions

Question 3: at pH = 7 Aspartate and Glutamate are______ charged

? Lysine and Arginine are________ charged ? ________ may be positively charged or uncharged

Answer:

negatively; positively; Histidine

MAKE SURE LECTURE 6 PAGE 8 MEMORIZE CHART IS IN THERE

Question 4: proteins are made of polypeptides, but polypeptides are NOT made of protein. T or F

Answer:

true

Question 5: protein family involved in oxygen binding proteins are ______

Answer:

globins Question 6: Three Chromatographical Methods for Protein Purification:

Answer:

ion exchange, size exclusion, affinity

Question 7: CO fits in the same binding site as O2.

? CO binds Hb 250 times better than O2.? CO is highly toxic. It blocks the function of myoglobin, hemoglobin, and others.

Answer:

CO poisioning Question 8: chaperone proteins are present in organisms franging from bacteria to humans.they require energy in the form of _______ to function because thermodynamic prefers randomness, and going from misfolded to a more organized correct fold requires energy

Answer:

ATP

Question 9: building blocks of proteins

Answer:

amino acids Question 10: part of metabolism that is oxidative, destructive, and releases energy ; goes from a complex thing (stored nutrients) to a small molecule; energy released as ATP or NADPH

Answer:

catabolism

Question 11: myoglobin is a monomers, so can it have quaternary structure?

Answer:

no Question 12: ________motions ("breathing") produce transient cavities in myoglobin for oxygen to move in and out.? The distal His (His E7 or His64) facilitates O2 binding by forming a hydrogen bond.

Answer:

Molecular Question 13: When a reaction is not at equilibrium, the driving force that moves the reaction towards equilibrium is expressed quantitatively as the free energy change for the reaction,

_____.

Answer:

?G

Question 14: Enzymes Lower the _____ ______ to Increase the Reaction Rate

Answer:

Activation Energy Question 15: Positive co-operativity can be recognized by_______ binding curves.

Answer:

sigmoidal Question 16: what are the subunit interactions in hemoglobin for the 4 subunits (alpha 1, alpha 2, beta 1, beta 2 = tetramer)

Answer:

hydrophobic, hydrogen bonds, and ionic interactions

Question 17: alkaline conditions ? [H+] lower ? [OH-] higher... pH is what?

Answer:

pH > 7

Question 18: catabolism is oxidative and degradative; it generates _______ and reduces

________

Answer:

ATP; coenzymes ; lecture 2 page 1 catabolism

Question 19: ion exchange chromatography - AnionExchange

• Matrix (____)

charged.

• Proteins (____) bind.

3. Elution: High salt,________ conditions.

Answer:

• (positively) ; negative; acidic - bc column is positive, protein are negative, want to pull protein from

positive matrix, the acidic solution gives more protons and helps protein fall off the matrix Question 20: alpha helix is a Very________ structure:

? Inner diameter: 4 - 5

A?, too small for anything to fit 'inside' ? Outer diameter (with

side chains): 10 - 12 A?, fits into dsDNA major grove

Answer:

compact Question 21: in a reducing agent, a disulfide bond would be _______ in cystine to form 2 cysteine amino acids with sulflhydryl bonds

Answer:

broken Question 22: living matter - High degree of________ at molecular and organismal levels.

Answer:

complexity