MB ASCP EXAM WITH CORRECT ACTUAL AND

MB (ASCP) EXAM WITH CORRECT ACTUAL AND

CORRECTLY WELL -DEFINED ANSWERS LATEST

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What is necessary to make cDNA? Correct Answer Reverse transcriptase

Number of SNPs in a human genome? Correct Answer 11 billion

(10^7)

How many genes (base pairs) are in a human haploid genome?Correct Answer 3 billion base pairs

The polymerization of acrylamide requires: Correct Answer

Catalyst (APS or TEMED) or Light activation

How does agarose gel pore size relate to concentration? Correct Answer Inversely proportional

What is denatured during a southern blot? Correct Answer Hydrogen bonds (inter-strand bonds)

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Melting temperature formula Correct Answer Tm = (4C x GC Pairs) + (2C x AT pairs)

Which is likely to interfere with fluorescent probe? Correct Answer 5' G

Proper GC content of primer Correct Answer 35-65%

Inhibitors of PCR Correct Answer Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol Blue

Order to add to a PCR reaction: Correct Answer Master mix >

patient sample > positive control > negative control

Pre Area Correct Answer Positive Pressure

Post Area Correct Answer Negative Pressure

Which PCR requires two steps for specificity? Correct Answer Nested PCR

Which microorganism is detected by branched DNA amplification?Correct Answer DNA or RNA

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Which technique uses RNA, antibodies, and DNA? Correct Answer Hybrid Capture Assay

Use of bisulfite treatment of DNA to determine its pattern of methylation Correct Answer Bisulfite DNA

What tests are isothermal? Correct Answer NASBA, TMA, & SDA

Which leukemia is associated with FLT3 gene? Correct Answer AML

T-cell gene rearrangement is seen in what malignancy? Correct Answer Sezary Syndrome

Which translocation causes Acute Premyelocytic Leukemia?Correct Answer t 15;17

Ewing Sarcoma Correct Answer t 11;22

Synovial Sarcoma Correct Answer t X;18

Follicular Lymphoma Correct Answer t 14;18

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