Test Bank for Biochemistry A

Test Bank for Biochemistry A Short Course 5 th Edition By Justin Hines, Catherine Reinke, John Tymoczko (Macmillan) (All Chapters 1- 41, 100% Original Verified, A+ Grade) All Chapters Arranged

Reverse: 41-1

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Page 1 Chapter 41

• What is the biological role of restriction enzymes in bacteria?

a.r epair DNA

• induce DNA crossover

c.c leave foreign DNA

• extract genes from the

bacteria

• open sticky ends

ANSWER:

c

• What is DNA cloning?

a.g rowing bacterial cells

• amplifying DNA

c.g rowing cellular cultures

• finding palindromic sequences

e.I dentical copies of the gene of interest

ANSWER:

e

• What is the direction of reverse transcription?

a.f rom 5′-phosphate to 3′-hydroxyl end

• from 3′-hydroxyl to 5′-phosphate

end c.f rom 5′-hydroxyl to 3′-phosphate end

• from 3′-phosphate to 5′-hydroxyl

end

• depends on the primer

ANSWER:

a

4. Recombinant DNA technologies are possible thanks to:

a.r estriction endonucleases.

• reverse transcriptases.

c.D NA polymerases.

• zinc-finger nucleases.

e.l igases.

ANSWER:

a

• DNA degradation by restriction enzyme at a specific site would be prevented by:

a.t wo-fold rotational symmetry.

• DNA sequence larger than 20 kb.

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Page 2 Chapter 41

• blunt ends of the DNA sequence.
• methylated cytosine base.

ANSWER:

e

• Restriction enzymes are useful in recombinant DNA technologies, as they allow:

a.g ene extraction from the bacteria.

• DNA to be released from the chromatin.

c.t o obtain smaller and more specific DNA fragments.

• cleavage of methylated DNA.

e.o btaining the entire genome from the cell.

ANSWER:

c

• What is the minimal amount of DNA that can be analyzed with gel electrophoresis?

a.5 0 ng

• 100 ng

c.5 0 ng/ml

• 5 mg

e.5µg

ANSWER:

a

• Which method allows generating DNA fragments with a determined length?

a.s creening of the expression vectors

• constructing a plasmid

c.g el-electrophoresis after reverse transcription

• Sanger dideoxy method

e.D NA cloning

ANSWER:

d

• What is a cDNA library?

a.collection of vectors made of restriction fragments b.collection of all mRNA of bacterial organisms c.collection of complementary DNA copies of mRNA from tissues or organisms, which are inserted into vectors for further studies d.collection of linker DNA e.collection of sequences made of DNA fragments

ANSWER:

c

• What is used as a primer for the synthesis of the first strand of cDNA?

a.o ligo(A) sequence

• the sequence complementary to the poly(A) tail

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Page 3 Chapter 41 c.o ligo (dC) sequence

• oligo (dG) sequence

e.t he sequence complementary to the 5′ cap

ANSWER:

b

11.R everse transcriptase is normally found in:

a.p lants.

• retroviruses.

c.m itochondria.

• the pancreas.

e.m ammals.

ANSWER:

b

• What is a genomic library?

a.c ollection of vectors made of restriction fragments

• collection of all mRNAs of some organisms

c.c ollection of vectors made of reversely transcribed mRNA

• collection of linker DNA

e.c ollection of sequences made of DNA fragments

ANSWER:

e

• Which method can amplify DNA sequences?

a.c ontrolled termination of replication

• polymerase chain reaction

c.s creening of the expression vectors

• constructing a plasmid

e.p olymerase cloning reaction

ANSWER:

b

• Which technique is BEST to study levels of gene expression in multiple genes and tissues?

a.q uantitative PCR

• gene cloning

c.g el electrophoresis

• DNA microarray

e.m icroinjections

ANSWER:

d

• What is a southern blotting technique?

a.t ransfer of DNA molecules on the nitrocellulose sheet

• autoradiographic detection of specifically selected molecules on the

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