VTNE Laboratory Exam Prep

VTNE Laboratory Exam Prep GRADED A+ (ACTUAL EXAM ) Questions and Answers (Solved)

• You are viewing a blood sample in a compound microscope at a 20X objective

lens. This microscope (as with most compound microscopes) has an ocular lens that has 10X magnification. What is the total magnification at which you are viewing the sample?

Answer: 200x

• Which of the following is the scabies mite in cats?

Notoedres cati Cheyletiella blakei Cuterebra Sarcoptes gati

Answer: Notoedres cati

Feline scabies is caused by the mite Notoedres cati. It often causes scaly or scabby facial lesions in cats and is intensely pruritic. It is a highly contagious mite and is closely related to the canine scabies mite Sarcoptes scabiei. It is diagnosed via a skin scraping.Demodex gati is the demodex cat mite. Sarcoptes gati is not a real mite. Cuterebra is a fly larva that may infest the skin of domestic animals. Cheyletiella is a surface mite that causes itchy and flaking skin in dogs and cats, also known as "walking dandruff".

• A cat may have ingested ethylene glycol. You are examining the urine sediment

and are looking for:

Answer: Antifreeze poisoning causes the formation of calcium oxalate crystals in the urine. White blood cells when seen are usually a sign of a urinary tract infection, but may also be associated with other disease processes occasionally. Struvite crystals are not uncommon to find in urine samples. Cystine crystals, although not commonly seen, in urine samples do not necessarily imply there is a problem.

• Which of the following is not an anticipated finding when evaluating a urine

sample after performing a cystocentesis?Struvite crystals Calcium oxalate crystals 5-10 white blood cells per high-powered field Erythrocytes Answer: There should be less than 0-5 white blood cells per high-powered field in a urine sample.

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Erythrocytes are not uncommon when performing a cystocentesis. This can occur secondary to trauma to the bladder wall when performing urine collection. Keep in mind that an excessive presence of blood can be abnormal.

Struvite and calcium oxalate crystaluria is not necessarily abnormal. Next Question

• A fresh biopsy sample from a nasal lesion is pressed against a glass slide several

times to leave an impression. The cells that are on the slide are stained with a Romanowsky stain and viewed under a microscope. Which of the following tests is this an example of?Immunohistochemistry Histology Immunocytochemistry Cytology Histopathology Answer: This is an example of cytology (referring to the examination of cells from body tissues or fluids). Cytology specimens may be collected by impression smear as described in this question or by other techniques including needle aspirate, aspiration of fluid (centesis), or by swab with a cotton-tipped applicator. Histology in- volves the examination of complete tissue and architecture and is usually performed on tissue collected by biopsy and processed intact. Histopathology is the same except that it involves the assessment of diseased tissue. Immunocytochemistry involves using special stains that are taken up by specific cellular components or markers on a cytologic specimen. Immunohistochemistry involves similar special stains applied to histologic sections.

• A patient needs to be fasted for which of the following blood test?

Answer: For a bile acids test, a fasted blood sample is drawn, the patient is fed, and another sample is drawn 2 hours after feeding (post-prandial).

• An elevated PCV is considered normal for which of the following breeds?

Akita Chihuahua Greyhound 2 / 3

Cavalier King Charles Spaniel Answer: Due to their sporting nature, Greyhounds tend to have packed cell volumes that are normally higher than other breeds.

• Which of these cell types are all typically found in circulation?

Leukocytes, thrombocytes, metamyelocytes Rubriblasts, thrombocytes, erythrocytes Lymphoblasts, erythrocytes, leukocytes Erythrocytes, leukocytes, thrombocytes Answer: The cellular makeup of the blood includes erythrocytes (red blood cells), leukocytes (white blood cells), and thrombocytes (platelets)

• Which of the following is a measure of red blood cell size on a complete blood

count?Mean Corpuscular Hemoglobin (MCH) Hematocrit Mean Corpuscular Volume (MCV)

Mean Corpuscular Hemoglobin Concentration (MCHC) Answer: Red blood cell size (in femtoliters) is described by the MCV. This value can be determined electronically by automated analyzers or manually by dividing the PCV by the RBC concentration and multiplying by 10.

• What is Mycology the study of?

Answer: Fungi

• A red blood cell fragment, as might be seen from shearing of the cell by trauma

in an animal with disseminated intravascular coagulopathy, is known as what?Spherocyte Echinocyte Acanthocyte Keratocyte Schistocyte Answer: A schistocyte is a red blood cell fragment that is usually formed due to shearing from intravascular trauma; they are seen in cases of DIC or vascular

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