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BIOLl 322/l BIOL322l Modulel 3:l (Latestl
2026/l 2027l Update)l 100%l Verifiedl Questionsl &l Answersl |l Gradel A
Q:l Whatl conclusionl didl Santiagol Ramonl Yl Cajall comel tol regardingl Golgil staining?l Whatl wasl thisl ideal called?
Answer:l
Cajall arrivedl atl al differentl conclusion:l thel nervousl systeml isl notl al giantl net,l butl ratherl al seriesl ofl individuall unitsl thatl arel separatedl froml onel anotherl physically.l Ideal wasl deemedl thel Neuronl Doctrine
Q:l Whichl idea,l thel Reticularl theory,l orl Neuronl doctrine,l representsl thel currentl understandingl ofl howl thel nervousl systeml isl organizedl today?
Answer:l
Thel Neuronl doctrine
Q:l Anotherl importantl stainl usedl tol distinguishl individuall cellsl inl nervousl tissuel isl the?l Whatl celll partsl doesl itl stain?
Answer:l
Nissll stain;l Stainsl RNAl andl DNAl (ONLYl localizedl tol neuronall celll bodies)
Q:l Al modificationl ofl thel stainingl methodsl thatl givesl increasedl specificityl overl thel otherl chemicall stainl methods?
Answer:l
Immunohistochemistryl (IHC)
Q:l Whyl isl IHCl useful?l (2) 1 / 3
Answer:l
1.l Itl isl goodl forl identifyingl thel locationl ofl specificl proteinsl atl al subcellularl level 2.l Itl canl bel usedl tol figurel outl wherel specificl neuronsl arel locatedl insidel orl onl thel cell
Q:l Whatl dol IHCl ultilize?l Whatl responsel dol thesel proteinsl functionl in?
Answer:l
Antibodies,l proteinsl thatl functionl inl thel immunel response
Q:l Eachl antibodyl hasl al partl ofl theirl Y-shapedl structurel thatl isl ablel tol bindl tol otherl targets,l whatl arel thesel targetsl called?
Answer:l
Antigens
Q:l Primaryl antibodyl vsl secondary
Answer:l
Primaryl -l abilityl tol bindl tol thel antigenl ofl interestl beingl studied Secondaryl -l targetsl isl somethingl thatl isl foundl onl thel primaryl antibody
Q:l Thel usel ofl antibodiesl andl fluorescencel microscopyl tol visualizel thel elementsl ofl cellsl inl al culturel dishl (inl vitro)
Answer:l
Immunocytochemistry
Q:l Onel ofl thel mainl difficultiesl withl immunochemistryl isl thatl thel antibodiesl oftenl exhibit.
Thisl canl alsol causel anl increasel inl what?
Answer:l
Nonspecificl bindingl 2 / 3
Backgroundl noise
Q:l Asl anl additionall feature,l thel secondaryl antibodyl isl conjugatedl withl al light- sensitivel .....,l orl light-producingl molecule
Answer:l
Fluorophore
Q:l Reliesl onl X-rayl technologyl thatl wasl developedl inl thel 1895
Answer:l
Computerizedl tomographyl (CT)l scan
Q:l High-energyl beamsl ofl electromagneticl radiationl thatl arel capablel ofl passingl throughl manyl physicall objects
Answer:l
X-rays
Q:l Materiall thatl isl morel densel showsl upl asl whatl colorl inl anl X-ray?l Lessl desne?
Answer:l
Morel densel -l white Lessl densel -l grey
Q:l Al three-dimensionall X-rayl thatl revolvesl aroundl thel personl asl theyl movel throughl thel scanner;l usesl al computerl thatl detectsl thel passagel ofl X-rays,l locatedl directlyl acrossl thel emissionl sourcel ofl thel X-ray
Answer:l
CTl scan
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