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DNA Analyst Certification Exam Practice

Class notes Jan 2, 2026 ★★★★☆ (4.0/5)
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DNA Analyst Certification Exam Practice Questions And Correct Answers (Verified Answers) Plus Rationales 2026 Q&A | Instant Download Pdf

  • Which enzyme is primarily used to cut DNA at specific sequences in
  • forensic analysis?

  • DNA polymerase
  • Restriction endonuclease
  • Ligase
  • Helicase

Rationale: Restriction endonucleases cut DNA at specific nucleotide

sequences, which is fundamental in DNA profiling.

  • What is the main purpose of the Polymerase Chain Reaction (PCR) in
  • forensic DNA analysis?

  • Separate DNA strands
  • Amplify small DNA samples 1 / 4
  • Sequence DNA
  • Stain DNA

Rationale: PCR allows forensic scientists to make millions of copies of a

small DNA sample for analysis.

  • STRs (Short Tandem Repeats) are most useful in forensic DNA profiling

because they:

  • Are identical in all humans
  • Vary greatly between individuals
  • Only appear on the Y chromosome
  • Are highly conserved

Rationale: STRs vary significantly between individuals, making them ideal

markers for identification.

4. In forensic DNA analysis, the term “allele” refers to:

  • A chromosome
  • A variant form of a gene at a specific locus
  • An entire genome
  • A DNA replication enzyme

Rationale: Alleles are different versions of a gene located at the same locus

on a chromosome.

  • Which of the following is a common source of DNA in forensic
  • samples?

  • Sweat 2 / 4
  • Hair shaft
  • Blood
  • Oil

Rationale: Blood contains nucleated cells and is a rich source of DNA for

analysis.

  • What is the purpose of electrophoresis in DNA analysis?
  • Amplify DNA
  • Sequence DNA
  • Separate DNA fragments by size
  • Denature proteins

Rationale: Electrophoresis separates DNA fragments based on size,

allowing visualization and comparison.

  • Which part of the DNA molecule is used to distinguish between
  • individuals?

  • Phosphate backbone
  • Non-coding regions
  • Hydrogen bonds
  • Ribose sugar

Rationale: Non-coding regions, including STRs, show variability between

individuals, which is used in profiling.

  • Which term describes a DNA sample that has been contaminated or
  • degraded? 3 / 4

  • High quality
  • Purified
  • Low template DNA
  • Plasmid DNA

Rationale: Low template DNA refers to samples with minimal or degraded

DNA, which require careful handling.

9. Mitochondrial DNA is especially useful in forensic cases when:

  • Nuclear DNA is abundant
  • Only degraded or hair samples without a root are available
  • Blood samples are present
  • STR analysis is possible

Rationale: Mitochondrial DNA is more stable and can be used when

nuclear DNA is insufficient or degraded.

10. Y-STR analysis is used primarily to:

  • Identify mitochondrial DNA
  • Amplify autosomal DNA
  • Trace male lineage
  • Determine hair color

Rationale: Y-STRs are located on the Y chromosome and are used to track

male-specific lineage.

11. The term “locus” in DNA profiling refers to:

  • DNA base pair
  • / 4

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Category: Class notes
Added: Jan 2, 2026
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DNA Analyst Certification Exam Practice Questions And Correct Answers (Verified Answers) Plus Rationales 2026 Q&A | Instant Download Pdf 1. Which enzyme is primarily used to cut DNA at specific seq...

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