UAMS IMMUNOLOGY ACTUAL EXAM 1 QUESTIONS

UAMS IMMUNOLOGY (ACTUAL / ) EXAM 1 QUESTIONS

AND VERIFIED ANSWERS

light scatter and specific abs tagged with fluorescent dyes - -- -Answers---how does flow cytometry ID cells?

through density gradient centrifugation with Ficoll-Hypaque - - --Answers---how are lymphocytes obtained for flow cytometry?

forward: size

side: granularity

and monoclonal abs labeled with fluorescent dye - --- Answers---how does light scatter in flow cytometry ID cells?

laser (typically argon) - ---Answers---what is the light source in light scatter flow cytometry?

fluorescence method - ---Answers---what method is used to screen for subpopulations such as B, Th, or Tc cells?

fluorescein isothiocyanate (FITC): green

phycoerythrin (PE): red to orange - ---Answers---what are

the most commonly used fluorochromes in the fluorescence method of flow cytometry? 1 / 3

fluorescense assisted cell sorting (FACS) - ---Answers--- what kind of flow cytometer can sort cells?

cell flows through beam, data sent to analyzer, computer signals charging coller to administer pos or neg charge, vibration breaks stream fo droplets (droplet is charged, cell not affected), droplet passes through 2 charged plates (pos attracts neg) (neg attracts pos) (uncharged is waste) - --- Answers---what is the principle of the FACS (cell sorter)?

CD45 (>95%) - ---Answers---what is the determinant marker and quantity of leukocytes?

CD34 (<1%) - ---Answers---what is the determinant marker and quantity of stem cells?

CD19-23 (10-15%) - ---Answers---what is the determinant marker and quantity of B cells?

CD2,3,5,7 (70% +/- 10) - ---Answers---what is the determinant marker anr quantity of T cells?

CD4 (50% +/- 5) - ---Answers---what is the determinant marker and quantity of helper T cells?

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CD8 (25% +/- 5) - ---Answers---what is the determinant marker and quantity of cytotoxic T cells?

(> or equal to 1) to 1 - ---Answers---what is the normal CD4 to CD8 ratio?

CD16 and CD56 (10-15%) - ---Answers---what is the determinant marker and quantity of NK cells?

total WBC x (% lymphs/100)= answer in /uL - ---Answers--- what is the formula for calculating the absolute lymph count?

absolute lymph count x (% CD marker/100)= answer in /uL - -- -Answers---what is the formula for calculating the absolute cell counts for T cells, Th cells, Tc cells, B cells, and Nk cells?

1.) vasodilation: chemical mediators are released are released

from injured cells; leads to redness and heat

2.) increased vascular permeability: caused by retraction of

endothelial cells lining blood vessles; allows fluid in plasma to leak to tissues; produces swelling and pain

3.) influx phagocytes: facilitated by more capillary

permeability; PMNs arrive first followed by macros - --- Answers---what is the sequence of inflammation?

neutrophil (PMNs) (most common) - ---Answers---what cells are involved in acute inflammation?

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